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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-AMFR antibody
See all AMFR products (5) ...
Description
Mouse monoclonal to AMFR
Tested applications
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
Recombinant fragment: QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL QLTRSVEITT DNILEGRIQV PFPTQRSDSI RPALNSPVER PSSDQEEGET SAQTERVPLD LSPRLEETLD , corresponding to amino acids 451-551 of Human AMFR
QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL QLTRSVEITT DNILEGRIQV PFPTQRSDSI RPALNSPVER PSSDQEEGET SAQTERVPLD LSPRLEETLD
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Western blot - AMFR antibody (ab54787)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 CUE domain.
Contains 1 RING-type zinc finger.
Cellular localization
Endoplasmic reticulum membrane.
Target information above from: UniProt accessionQ9UKV5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- isoform 2 antibody
- AMF receptor antibody
- AMF receptor isoform 1 antibody
see all
Database links
- Entrez Gene: 267 Human
- Omim: 603243 Human
- SwissProt: P26442 Human
- SwissProt: Q9UKV5 Human
- Unigene: 295137 Human
Anti-AMFR antibody images:
Western blot - AMFR antibody (ab54787)
Western blot against tagged recombinant protein immunogen using ab54787 AMFR antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
References for Anti-AMFR antibody (ab54787)
This product has been referenced in:
- Joshi Bet al. A role for KAI1 in promotion of cell proliferation and mammary gland hyperplasia by the gp78 ubiquitin ligase. J Biol Chem 285:8830-9 (2010). WB; Mouse.Read more (PubMed: 20089858) »
See 1 publication for this product
Publishing research using ab54787? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"