You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-AMFR antibody (ab58782)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-AMFR antibody
See all AMFR products (5) ...

Description

Rabbit polyclonal to AMFR

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog, Zebrafish

Immunogen

a region within synthetic peptide: SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ, corresponding to C terminal amino acids 541-590 of Human AMFR

SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ

Positive control

HepG2 cell lysate.

Properties

Form

Lyophilised:
Add 100 µl of distilled water for a final concentration of 1mg/ml.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - AMFR antibody (ab58782)Western blot - AMFR antibody (ab58782) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500
WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 34 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Contains 1 CUE domain.
Contains 1 RING-type zinc finger.

Cellular localization

Endoplasmic reticulum membrane.

Target information above from: UniProt accessionQ9UKV5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • isoform 2 antibody
  • AMF receptor antibody
  • AMF receptor isoform 1 antibody
  • AMF receptor isoform 2 antibody
  • AMFR antibody
  • AMFR2_HUMAN antibody
  • Autocrine motility factor receptor antibody
  • Autocrine motility factor receptor precursor, isoform 1 antibody
  • Autocrine motility factor receptor precursor, isoform 2 antibody
  • E3 ubiquitin-protein ligase AMFR antibody
  • gp78 antibody
  • RING finger protein 45 antibody
  • RNF 45 antibody
  • RNF45 antibody
see all

Anti-AMFR antibody images:

  Western blot - AMFR antibody (ab58782)

Western blot - AMFR antibody (ab58782)

Anti-AMFR antibody (ab58782) at 2.5 µg/ml + HepG2 cell lysate at 10 µg/ml with 5% skim milk in PBS buffer

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 34 kDa


Gel concentration: 12%

References for Anti-AMFR antibody (ab58782)

ab58782 has not yet been referenced specifically in any publications.

Publishing research using ab58782? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"