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Western blot - AMFR antibody (ab58782)
(enlarge)
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Product Name
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AMFR antibody
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See all AMFR antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AMFR
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Immunogen
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a region within synthetic peptide: SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ, corresponding to C terminal amino acids 541-590 of Human AMFR
BLAST 'SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500
WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate.
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Cellular localization
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Single-pass type I membrane protein.
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Research areas
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Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Relevance
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AMFR (Autocrine motility factor receptor, isoform 2) is specific receptor for the autocrine motility factor. AMFR possesses ubiquitin ligase activity and can target both itself and other proteins including CD3D and APOB for proteasomal degradation. AMFR mediates tumor invasion and metastasis. The protein interacts with UBE2G2/UBC7 through a region distinct from the RING finger.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 100 µl of distilled water for a final concentration of 1mg/ml.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AMFR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AMFR antibody images, references, products related to ab58782 and other tools.
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AMFR antibody images:
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 Western blot - AMFR antibody (ab58782)
AMFR antibody (ab58782) at 2.5 µg/ml + HepG2 cell lysate at 10 µg/ml with 5% skim milk in PBS buffer
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 34 kDa
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to AMFR
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AMFR antibody - more information
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Customer reviews (feedback) regarding AMFR antibody |
Customer FAQs regarding AMFR antibody |
Protocols for AMFR antibody |
Price and availability of products related to AMFR antibody |
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AMFR antibody - related products:
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AMFR antibody - other tools:
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Contact Abcam with a Technical Enquiry about AMFR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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