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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-AMFR antibody
See all AMFR products (5) ...
Description
Rabbit polyclonal to AMFR
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
a region within synthetic peptide: SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ, corresponding to C terminal amino acids 541-590 of Human AMFR
SPRLEETLDF GEVEVEPSEV EDFEARGSRF SKSADERQRM LVQRKDELLQ
Positive control
HepG2 cell lysate.
Properties
Form
Lyophilised:
Add 100 µl of distilled water for a final concentration of 1mg/ml.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Western blot - AMFR antibody (ab58782)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500
WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 CUE domain.
Contains 1 RING-type zinc finger.
Cellular localization
Endoplasmic reticulum membrane.
Target information above from: UniProt accessionQ9UKV5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- isoform 2 antibody
- AMF receptor antibody
- AMF receptor isoform 1 antibody
see all
Database links
- Entrez Gene: 267 Human
- Entrez Gene: 23802 Mouse
- Entrez Gene: 361367 Rat
- Omim: 603243 Human
- SwissProt: P26442 Human
- SwissProt: Q9UKV5 Human
- SwissProt: Q9R049 Mouse
- Unigene: 295137 Human
see all
Anti-AMFR antibody images:
Western blot - AMFR antibody (ab58782)
Anti-AMFR antibody (ab58782) at 2.5 µg/ml + HepG2 cell lysate at 10 µg/ml with 5% skim milk in PBS buffer
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 34 kDa
Gel concentration: 12%
References for Anti-AMFR antibody (ab58782)
ab58782 has not yet been referenced specifically in any publications.
Publishing research using ab58782? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"