Anti-AMIGO1 antibody [S86-36] (ab167439)
Key features and details
- Mouse monoclonal [S86-36] to AMIGO1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-AMIGO1 antibody [S86-36] -
Description
Mouse monoclonal [S86-36] to AMIGO1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human AMIGO1 aa 350-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (cytoplasmic C-terminus)
Database link: Q80ZD8 -
Positive control
- WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S86-36 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 55 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 55 kDa). |
ICC/IF
1/100. |
Target
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Function
Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. -
Sequence similarities
Belongs to the immunoglobulin superfamily. AMIGO family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Domain
The LRR repeat region mediates homodimerization. -
Cellular localization
Cell membrane. Cell projection > axon. Associated with axons of neuronal cells. - Information by UniProt
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Database links
- Entrez Gene: 57463 Human
- Entrez Gene: 295365 Rat
- SwissProt: Q86WK6 Human
- SwissProt: Q80ZD7 Rat
- Unigene: 726479 Human
- Unigene: 116338 Rat
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Alternative names
- Adhesion molecule with Ig like domain 1 antibody
- ali2 antibody
- Alivin 2 antibody
see all
Images
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Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg
Secondary
Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skim Milk in 1X TBST.
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4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab167439 has not yet been referenced specifically in any publications.