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- Secondary antibodies
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Product name
Anti-AMPK alpha 1 + AMPK alpha 2 antibody
See all AMPK alpha 1 + AMPK alpha 2 products (7) ...
Description
Rabbit polyclonal to AMPK alpha 1 + AMPK alpha 2
Specificity
ab39644 detects endogenous levels of total AMPK alpha 1/AMPK alpha 2 protein.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (non-phosphorylated) derived from human AMPK alpha 1/AMPK alpha 2 around the phosphorylation site of Serine 485 or Serine 491.
Positive control
Human colon carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 + AMPK alpha 2 antibody (ab39644)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
Target
Relevance
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Cellular localization
Cytoplasmic
Alternative names
- 5' AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic alpha 2 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 602739 Human
- Omim: 600497 Human
see all
Anti-AMPK alpha 1 + AMPK alpha 2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 + AMPK alpha 2 antibody (ab39644)
ab39644, at 1/50 dilution, staining AMPK alpha 1 + AMPK alpha 2 in Human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue.
References for Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab39644)
ab39644 has not yet been referenced specifically in any publications.
Publishing research using ab39644? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"