Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody
See all AMPK alpha 1 + AMPK alpha 2 products (7) ...
Description
Rabbit polyclonal to AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491)
Specificity
ab39400 detects endogenous levels of AMPK alpha 1 + AMPK alpha 2 only when phosphorylated at Serine 485 or Serine 491.
Tested applications
IHC-Fr, ICC/IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of Serine 485/491.
Positive control
IHC: human colon carcinoma WB: lysate of HeLa cells treated with heat shock
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
-
Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
(enlarge)
-
Immunohistochemistry (Frozen sections) - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
(enlarge)
-
Immunocytochemistry/ Immunofluorescence - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
WB: 1/5000 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Relevance
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Cellular localization
Cytoplasmic
Alternative names
- 5' AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic alpha 2 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 602739 Human
- Omim: 600497 Human
see all
Anti-AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
Immunohistochemical analysis of paraffin embedded human colon carcinoma, using ab39400 at a 1/100 dilution. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunohistochemistry (Frozen sections) - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
ab39400 staining AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) in mouse liver tissue sections by IHC-Fr (formaldehyde-fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with 2% BSA for 30 minutes at 20°C. The sample was incubated with primary antibody (1/200 in PBS) at 4°C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/200) was used as secondary antibody. Nuclei were stained with DAPI.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
ab39400 staining AMPK alpha 1 + AMPK alpha 2 in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
This image is courtesy of an anonymous abreview.
Western blot - AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
Anti-AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400) at 1/2000 dilution (in PBS-T for 12 hours at 4°C) + Whole cell lysate of mouse liver at 50 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 62 kDa
Exposure time : 30 seconds
Blocking Step: 5% Milk for 2 hours at 25°C
This image is courtesy of an anonymous Abreview
References for Anti-AMPK alpha 1 + AMPK alpha 2 (phospho S485 + S491) antibody (ab39400)
ab39400 has not yet been referenced specifically in any publications.
Publishing research using ab39400? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"