Anti-AMPK alpha 1 antibody [Y365] (ab32047)

Overview

• Product nameAnti-AMPK alpha 1 antibody [Y365]See all AMPK alpha 1 primary antibodies ...
• Description
  Rabbit monoclonal [Y365] to AMPK alpha 1
• SpecificityThis antibody is specific for human AMPK alpha 1.
• Tested applicationsWB, IHC-P, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C-terminus of Human AMPK alpha 1 subunit.

• Positive controlHela cells and MCF-7 cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberY365
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Amyloid

Applications

Target

• FunctionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
  Contains 1 protein kinase domain.

Target information above from: UniProt accession Q13131 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5562 Human
  • Entrez Gene: 5557 Human
  • Entrez Gene: 5562 Human
  • Entrez Gene: 105787 Mouse
  • Entrez Gene: 65248 Rat
  • Omim: 602739 Human
  • SwissProt: Q13131 Human
  • SwissProt: Q13131 Human

  • SwissProt: P49642 Human
  • SwissProt: Q5EG47 Mouse
  • SwissProt: P54645 Rat
  • Unigene: 43322 Human
  • Unigene: 207004 Mouse
  • Unigene: 87789 Rat

  see all

• Alternative names
  5 AMP activated protein kinase alpha 1catalytic subunit antibody5 AMP activated protein kinase catalytic alpha 1 chain antibody5' AMP activated protein kinase catalytic subunit alpha 1 antibody

  5'-AMP-activated protein kinase catalytic subunit alpha-1 antibodyAAPK1 antibodyAAPK1_HUMAN antibodyacetyl CoA carboxylase kinase antibodyAI194361 antibodyAI450832 antibodyAL024255 antibodyAMP -activate kinase alpha 1 subunit antibodyAMP-activated protein kinase, catalytic, alpha -1 antibodyAMPK 1 antibodyAMPK alpha 1 antibodyAMPK alpha 1 chain antibodyAMPK antibodyAMPK subunit alpha-1 antibodyAMPK1 antibodyAMPKa1 antibodyAMPKalpha1 antibodyC130083N04Rik antibodycb116 antibodyEC 2.7.11.1 antibodyHMG CoA reductase kinase antibodyhormone sensitive lipase kinase antibodyim:7154392 antibodykinase AMPK alpha1 antibodyMGC33776 antibodyMGC57364 antibodyOTTHUMP00000161795 antibodyOTTHUMP00000161796 antibodyPRKAA 1 antibodyPRKAA1 antibodyProtein kinase AMP activated alpha 1 catalytic subunit antibodySNF1-like protein AMPK antibodyTau protein kinase PRKAA1 antibodywu:fa94c10 antibody

  see all

Anti-AMPK alpha 1 antibody [Y365] images

• 

  Western blot - AMPK alpha 1 antibody [Y365] (ab32047)
  All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/5000 dilution
  
  Lane 1 : MCF-7
  Lane 2 : Hela
  
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 antibody [Y365] (ab32047)
  ab32047 at a 1:100 - 1:250 dilution staining AMPK alpha 1 in Human cervical carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.