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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Overview
Product name
Anti-AMPK alpha 1 antibody
See all AMPK alpha 1 products (14) ...
Description
Rabbit polyclonal to AMPK alpha 1
Specificity
Does not cross-react with AMPK alpha 2.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Rat, Cow, Human
Immunogen
Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
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Western blot - AMPK alpha 1 antibody (ab3759)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at µg/mg of lysate. (Recommended to be used at a concentration of 2 - 8 µg/mg of lysate. )
WB: 1/500 - 1/5000.Predicted molecular weight: 63 kDa.
Target
Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionQ13131
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: P54645 Rat
- Unigene: 43322 Human
- Unigene: 87789 Rat
Anti-AMPK alpha 1 antibody images:
Western blot - AMPK alpha 1 antibody (ab3759)
Predicted band size : 63 kDa
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).
References for Anti-AMPK alpha 1 antibody (ab3759)
This product has been referenced in:
- Koo JS & Kim H Hypoxia-related protein expression and its clinicopathologic implication in carcinoma of unknown primary. Tumour Biol 32:893-904 (2011). IHC-P; Human.Read more (PubMed: 21598042) »
- Mayes MAet al. Adenosine 5'-monophosphate kinase-activated protein kinase (PRKA) activators delay meiotic resumption in porcine oocytes. Biol Reprod 76:589-97 (2007).Read more (PubMed: 17167165) »
See all 3 publications for this product
Publishing research using ab3759? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"