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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 products (10) ...
Description
Rabbit polyclonal to AMPK alpha 2
Specificity
This antibody recognizes human AMP activated protein kinase (AMPK) alpha 2.
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Horse, Cow, Dog, Pig
Immunogen
Synthetic peptide: CDDSAMHIPPGLKPHP conjugated to KLH, corresponding to amino acids 353-367 of Human AMPK alpha 2, with an N-terminal cysteine added for conjugation.
CDDSAMHIPP GLKPHP
Positive control
Rat liver.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide.
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4.
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 1 - 2 µg/ml. Dilute in 5% nonfat dry milk in PBS, 0.05% Tween 20. Incubate for 2 hours at room temperature. Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 63 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP54646
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5' AMP activated protein kinase catalytic alpha 2 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 2 antibody
- 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
see all
Database links
- Entrez Gene: 5563 Human
- Entrez Gene: 108079 Mouse
- Entrez Gene: 397504 Pig
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- SwissProt: P54646 Human
- SwissProt: Q8BRK8 Mouse
- SwissProt: Q28948 Pig
see all
References for Anti-AMPK alpha 2 antibody (ab31959)
This product has been referenced in:
- Moir Het al. AMPK inactivation in mononuclear cells: a potential intracellular mechanism for exercise-induced immunosuppression. Appl Physiol Nutr Metab 33:75-85 (2008). WB; Human.Read more (PubMed: 18347656) »
See 1 publication for this product
Publishing research using ab31959? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"