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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 products (10) ...
Rabbit polyclonal to AMPK alpha 2
Does not cross-react with AMPK alpha 1.
ICC/IF, WB, IP, IHC-Pmore details
Reacts with
Mouse, Rat, Cow, Human
Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7.
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab3760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/500 - 1/5000.Predicted molecular weight: 63 kDa.
IP: Use a concentration of 2 - 8 µg/ml.
IHC-P: Use at an assay dependent concentration.
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP54646
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - AMPK alpha 2 antibody (ab3760)

Predicted band size : 63 kDa
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 2 (ab3760) used at 2ug/ml (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK alpha 2 antibody (ab3760)

ab3760 staining AMPK alpha 2 in murine thymus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 2% BSA for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 18 hours at 4°C. An HRP-conjugated anti-rabbit was used as secondary antibody at a 1/50 dilution. ab3760 presents in both the nuclear and the cytoplasm in mouse thymus.
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - AMPK alpha 2 antibody (ab3760)

ICC/IF image of ab3760 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3760, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
See all 3 publications for this product
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Predicted band size : 63 kDa
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 2 (ab3760) used at 2ug/ml (1/500).

ab3760 staining AMPK alpha 2 in murine thymus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 2% BSA for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 18 hours at 4°C. An HRP-conjugated anti-rabbit was used as secondary antibody at a 1/50 dilution. ab3760 presents in both the nuclear and the cytoplasm in mouse thymus.
Image courtesy of an anonymous Abreview.

ICC/IF image of ab3760 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3760, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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