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Western blot - AMPK alpha 2 antibody (ab3760)

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Product Name
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AMPK alpha 2 antibody
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See all AMPK alpha 2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AMPK alpha 2
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Immunogen
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Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7.
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Reacts with
(species key)
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Hu, Ms, Rat, Cow
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Specificity
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Does not cross-react with AMPK alpha 1.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IP: Use at a concentration of 2 - 8 µg/ml. WB: 1/500 - 1/5000. Predicted molecular weight: 63 kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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The AMP activated protein kinase (AMPK) in mammals, and its homologue in Saccharomyces cerevisiae, are activated by cellular stresses associated with ATP depletion. AMPK is a heterotrimer comprising a catalytic alpha subunit with associated beta and gamma subunits. The alpha subunit has at least two isoforms (alpha 1 and alpha 2), which differ in their AMP dependence and subcellular localization, with alpha 2 complexes being partly nuclear. AMPK is activated allosterically by 5' AMP, which also promotes phosphorylation and activation by an upstream kinase, and inhibits dephosphorylation and inactivation. Elevation of AMP always accompanies depletion of ATP due to the action of adenylate kinase. Since high ATP antagonizes the activating effects of AMP, the system behaves like a cellular 'fuel gauge'. It is activated by various types of stress associated with ATP depletion, such as hypoxia, heat shock, metabolic poisoning and, in muscle, exercise. AMPK phosphorylates multiple targets which switch off anabolic pathways and switch on alternative catabolic pathways.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AMPK alpha 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AMPK alpha 2 antibody images, references, products related to ab3760 and other tools.
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AMPK alpha 2 antibody images:
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Western blot - AMPK alpha 2 antibody (ab3760)
Predicted band size : 63 kDa
Samples: Extracts from 1. Bovine aortic endothelial cells 2. Rat aortic smooth muscle cells 3. HepG2 cells 4. Human aortic endothelial cells Antibody: Affinity purified Rabbit anti-AMPK alpha 2 (ab3760) used at 2ug/ml (1/500).
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References for AMPK alpha 2 antibody (ab3760)
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This product has been used in: (two most recent references)
Lee-Young RS et al. Skeletal muscle AMP-activated protein kinase is essential for the metabolic response to exercise in vivo. J Biol Chem 284:23925-34 (2009). ICC/IF, IP, htPCR; Mouse. PubMed: 19525228
Sag D et al. Adenosine 5'-monophosphate-activated protein kinase promotes macrophage polarization to an anti-inflammatory functional phenotype. J Immunol 181:8633-41 (2008). WB; Human, Mouse. PubMed: 19050283
See all 3 publication references for this product.
If you publish research using ab3760 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AMPK alpha 2
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AMPK alpha 2 antibody - more information
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Customer reviews (feedback) regarding AMPK alpha 2 antibody |
Customer FAQs regarding AMPK alpha 2 antibody |
Protocols for AMPK alpha 2 antibody |
Price and availability of products related to AMPK alpha 2 antibody |
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AMPK alpha 2 antibody - related products:
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AMPK alpha 2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AMPK alpha 2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"