AMPK alpha 2 antibody (ab3760)

Product Name 

AMPK alpha 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AMPK alpha 2

Immunogen 

Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7.

Reacts with 
(species key)

Hu, Ms, Rat, Cow

Specificity 

Does not cross-react with AMPK alpha 1.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 2 - 8 µg/ml.
WB: 1/500 - 1/5000. Predicted molecular weight: 63 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The AMP activated protein kinase (AMPK) in mammals, and its homologue in Saccharomyces cerevisiae, are activated by cellular stresses associated with ATP depletion. AMPK is a heterotrimer comprising a catalytic alpha subunit with associated beta and gamma subunits. The alpha subunit has at least two isoforms (alpha 1 and alpha 2), which differ in their AMP dependence and subcellular localization, with alpha 2 complexes being partly nuclear. AMPK is activated allosterically by 5' AMP, which also promotes phosphorylation and activation by an upstream kinase, and inhibits dephosphorylation and inactivation. Elevation of AMP always accompanies depletion of ATP due to the action of adenylate kinase. Since high ATP antagonizes the activating effects of AMP, the system behaves like a cellular 'fuel gauge'. It is activated by various types of stress associated with ATP depletion, such as hypoxia, heat shock, metabolic poisoning and, in muscle, exercise. AMPK phosphorylates multiple targets which switch off anabolic pathways and switch on alternative catabolic pathways.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

See below for AMPK alpha 2 antibody images, references, products related to ab3760 and other tools.

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References for AMPK alpha 2 antibody (ab3760)

  This product has been used in: (two most recent references)

Lee-Young RS et al. Skeletal muscle AMP-activated protein kinase is essential for the metabolic response to exercise in vivo. J Biol Chem 284:23925-34 (2009). ICC/IF, IP, htPCR; Mouse. PubMed: 19525228

Sag D et al. Adenosine 5'-monophosphate-activated protein kinase promotes macrophage polarization to an anti-inflammatory functional phenotype. J Immunol 181:8633-41 (2008). WB; Human, Mouse. PubMed: 19050283

See all 3 publication references for this product.


If you publish research using ab3760 please let us know so that we can cite the reference on this datasheet.

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