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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 products (10) ...
Description
Mouse monoclonal to AMPK alpha 2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MSLQLYLVDN RSYLLDFKSI DDEVVEQRSG SSTPQRSCSA AGLHRPRSSF DSTTAESHSL SGSLTGSLTG STLSSVSPRL GSHTMDFFEM CASLITTLAR , corresponding to amino acids 453-553 of Human AMPK alpha 2
MSLQLYLVDN RSYLLDFKSI DDEVVEQRSG SSTPQRSCSA AGLHRPRSSF DSTTAESHSL SGSLTGSLTG STLSSVSPRL GSHTMDFFEM CASLITTLAR
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
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Western blot - AMPK alpha 2 antibody (ab58171)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP54646
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
- AAPK2_HUMAN antibody
- AMPK alpha 2 chain antibody
see all
Database links
- Entrez Gene: 5563 Human
- Omim: 600497 Human
- SwissProt: P54646 Human
- Unigene: 256067 Human
- Unigene: 437039 Human
Anti-AMPK alpha 2 antibody images:
Western blot - AMPK alpha 2 antibody (ab58171)
Western blot against tagged recombinant protein immunogen using ab58171 AMPK alpha 2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-AMPK alpha 2 antibody (ab58171)
ab58171 has not yet been referenced specifically in any publications.
Publishing research using ab58171? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"