Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922)

Overview

• Product nameAnti-AMPK beta 1 antibody - Aminoterminal endSee all AMPK beta 1 primary antibodies ...
• Description
  Rabbit polyclonal to AMPK beta 1 - Aminoterminal end
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A KLH conjugated synthetic peptide selected from the N terminal region of human AMPK beta 1.

• Positive controlHuman hepatocarcinoma tissue, Jurkat cell lysate and mouse spleen tissue lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Lipid Kinases

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

Applications

Target

• FunctionNon-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
• Sequence similaritiesBelongs to the 5'-AMP-activated protein kinase beta subunit family.
• DomainThe glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
• Post-translational
  modificationsPhosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

Target information above from: UniProt accession Q9Y478 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5564 Human
  • Entrez Gene: 19079 Mouse
  • Omim: 602740 Human
  • SwissProt: Q9Y478 Human
  • SwissProt: Q9R078 Mouse
  • Unigene: 726001 Human
  • Unigene: 6061 Human
  • Unigene: 458152 Mouse

• Alternative names
  1300015D22Rik antibody5 AMP activated protein kinase subunit beta 1 antibody5''-AMP-activated protein kinase subunit beta-1 antibody

  AAKB1_HUMAN antibodyAMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibodyAMP-activated, noncatalytic, beta-1 antibodyAMPK antibodyAMPK beta 1 chain antibodyAMPK subunit beta-1 antibodyAMPK-BETA-1 antibodyAMPKb antibodyAU021155 antibodyE430008F22 antibodyHAMPKb antibodyMGC17785 antibodyPRKAB1 antibody

  see all

Anti-AMPK beta 1 antibody - Aminoterminal end images

• 

  Western blot - AMPK beta 1 antibody - Aminoterminal end (ab70922)
  All lanes : Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922) at 1/100 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : Mouse spleen tissue lysate
  
  Lysates/proteins at 12.5 µg per lane.
  
  
  Predicted band size : 30 kDa
  Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 antibody - Aminoterminal end (ab70922)
  Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70922 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.