Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922)

Overview

  • Product nameAnti-AMPK beta 1 antibody - Aminoterminal end
    See all AMPK beta 1 primary antibodies
  • Description
    Rabbit polyclonal to AMPK beta 1 - Aminoterminal end
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N terminal region of human AMPK beta 1.

  • Positive control
    • Human hepatocarcinoma tissue, Jurkat cell lysate and mouse spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 32 kDa plus a range of other bands (predicted molecular weight: 30 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNon-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
    • Sequence similaritiesBelongs to the 5'-AMP-activated protein kinase beta subunit family.
    • DomainThe glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
    • Post-translational
      modifications
      Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1300015D22Rik antibody
      • 5 AMP activated protein kinase subunit beta 1 antibody
      • 5''-AMP-activated protein kinase subunit beta-1 antibody
      • AAKB1_HUMAN antibody
      • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
      • AMP-activated, noncatalytic, beta-1 antibody
      • AMPK antibody
      • AMPK beta 1 chain antibody
      • AMPK subunit beta-1 antibody
      • AMPK-BETA-1 antibody
      • AMPKb antibody
      • AU021155 antibody
      • E430008F22 antibody
      • HAMPKb antibody
      • MGC17785 antibody
      • PRKAB1 antibody
      see all

    Anti-AMPK beta 1 antibody - Aminoterminal end images

    • All lanes : Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922) at 1/100 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 12.5 µg per lane.


      Predicted band size : 30 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70922 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    References for Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922)

    ab70922 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"