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AMPK gamma 1 protein (Tagged-His Tag) (ab91948)

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Overview

  • Product nameAMPK gamma 1 protein (Tagged-His Tag)See all AMPK gamma 1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 40-247 (208 amino acids) of Human AMPK gamma 1 with N terminal His tag; MWt 26kDa (NP_997626.1).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 49 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNT LYILTHKRI LKFLKLFITEFPKPEFMSKSLEELQIGTY ANIAMVRTTTPVYVALGIFV QHRVSALPVVDEKGRVVD IYSKFDVINLAAEKTYNNLDVSVTKALQHRS HYFEGVL KCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSD IL QALVLTGGEKKP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91948 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AMP activated protein kinase noncatalytic gamma 1 subunitAMPK gamma 1 chainAMPKg
        MGC8666PRKAG 1PRKAG1Protein kinase AMP activated gamma 1 non catalytic subunit
      see all
  • RelevanceThe 5 AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.
  • Cellular localizationCytoplasmic

    • AMPK gamma 1 protein (Tagged-His Tag) images

    • SDS-PAGE - AMPK gamma 1 protein (Tagged-His Tag) (ab91948)
      SDS-PAGE - AMPK gamma 1 protein (Tagged-His Tag) (ab91948)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for AMPK gamma 1 protein (Tagged-His Tag) (ab91948)

    ab91948 has not yet been referenced specifically in any publications.

    Publishing research using ab91948 ? Please let us know so that we can cite the reference in this datasheet

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