Overview

  • Product name
  • Description
    Rabbit polyclonal to ANKH
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (DLGYYIINKL HHVDESVGSK TRRAFLYLAA FPFMDAMAWT HAGILLKHKY) of Human ANKH (NP_473368).

  • Positive control
    • THP-1 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/250.

Target

  • Function
    Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter.
  • Tissue specificity
    Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells.
  • Involvement in disease
    Chondrocalcinosis 2
    Craniometaphyseal dysplasia, autosomal dominant
  • Sequence similarities
    Belongs to the ANKH family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANK antibody
    • ANKH antibody
    • ANKH_HUMAN antibody
    • Ankylosis progressive homolog (mouse) antibody
    • Ankylosis progressive homolog antibody
    • CCAL 2 antibody
    • CCAL2 antibody
    • CMDJ antibody
    • CPPDD antibody
    • Craniometaphyseal dysplasia Jackson type (dominant) antibody
    • FLJ27166 antibody
    • HANK antibody
    • MANK antibody
    • Progressive ankylosis protein homolog antibody
    see all

Images

  • Anti-ANKH antibody (ab90104) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
  • ab90104 staining ANKH in Mouse pre-osteoblast MC3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin, permeabilized with TritonX and blocked with 3% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in 0.5% serum + PBS) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Du G  et al. Abnormal Mechanical Loading Induces Cartilage Degeneration by Accelerating Meniscus Hypertrophy and Mineralization After ACL Injuries In Vivo. Am J Sports Med 44:652-63 (2016). Read more (PubMed: 26792705) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Pre-osteoblast cell line MC3T3)
Specification
Pre-osteoblast cell line MC3T3
Fixative
Formaline
Permeabilization
Yes - TritonX
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

Submitted Jun 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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