ANP ELISA kit (ab108797)
- Product nameANP ELISA kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Tissue
- Assay typeSandwich
- Sensitivity= 0.2 ng/ml
- Range0.25 ng/ml - 4 ng/ml
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
- Product overview
Atrial natriuretic peptide (ANP), a 28 amino acid polypeptide, is mainly secreted from the atrium of the heart where it is stored in secretory granules as a 136 amino acid pro-hormone. Upon its secretion, induced by increases in atrial pressure and stretch, the pro-hormone is processed by a serine protease to the active 28 amino acid peptide. The peptide binds with high affinity to the membrane receptor guanylate cyclase GC-A, leading to increased intracellular cGMP levels. Increased ANP level in plasma has been identified as a predictor of cardiac dysfunction and prognosis in congestive heart failure and ischemic heart disease.
ab108797 ANP Rat ELISA kit is designed for detection of rat ANP in plasma, serum, tissue extract and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rANP in 4 hours. A polyclonal antibody specific for rANP has been pre-coated onto a microplate. The rANP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rANP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated rANP Antibody 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml rANP Microplate 1 x 96 tests rANP Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- ANFATFB6Atrial natriuretic factor
- Atrial natriuretic peptideAtriopeptinCardionatrinCDD ANFNatriuretic peptide ANPPAPNDPrepronatriodilatin
- SwissProt: P01160 Human
Our Abpromise guarantee covers the use of ab108797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for ANP ELISA kit (ab108797)
ab108797 has not yet been referenced specifically in any publications.