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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
AOS1+UBA2 protein
Properties
Purification notes
The Sumo E1 protein is a heterodimer consisting of human Aos 1 and Uba 2. Both the Aos 1 and Uba 2 are co-expressed in a baculovirus expression system, prepared from freshly extracted cell lysates, and purified to homogeneity. The final fraction of Aos 1 and Uba 2 is an affinity column pool.
Biological activity
The final fraction of enzyme contains two single polypeptide bands of 38 kDa and 80 kDa.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol
Concentration
Concentration information loading...
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Functional Studies
Application notes
Sumoylation assays are carried out in a final volume of 20 µl in reaction buffer (20 mM Hepes pH 7.5, 5mM MgCl2, 2mM ATP). We would recommend adding fresh 2mM ATP to be sure that sufficient energy is supplied.
Uba 2 contains his-rich regions which might cross-react with antibodies against the 6x-His epitope tag.
During western analysis, Uba 2 at 80 kDa might be shown.
Protein info
Relevance
E1-SUMO protein is a heterodimer consisted of human AOS1 and UBA2. The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Cellular localization
Nuclear
AOS1+UBA2 protein images:
Sumoylation assay
Left Lane: Topo I protein (Topo I fragment: ab3828)
Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Note: Topo I is S35-Met labeled.
References for AOS1+UBA2 protein (ab3804)
ab3804 has not yet been referenced specifically in any publications.
Publishing research using ab3804? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"