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Immunocytochemistry/ Immunofluorescence-AP2 alpha antibody(ab11828)
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Product Name
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AP2 alpha antibody
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See all AP2 alpha - transcription factor antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AP2 alpha
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Immunogen
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Synthetic peptide: SHTDNNAKSSDKEEKHRK conjugated to KLH, corresponding to amino acids 420-437 of Human AP2 alpha.
BLAST 'SHTDNNAKSSDKEEKHRK' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/100.
WB: 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Nuclear extract of HeLa human epithelioid carcinoma cells (western blotting) and of fixed cultured HeLa cells (immunofluorescence).
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AP2 alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AP2 alpha antibody images, references, products related to ab11828 and other tools.
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AP2 alpha antibody images:
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 Immunocytochemistry/ Immunofluorescence-AP2 alpha antibody(ab11828)
ICC/IF image of ab11828 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11828, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to AP2 alpha
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AP2 alpha antibody - more information
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Customer reviews (feedback) regarding AP2 alpha antibody |
Customer FAQs regarding AP2 alpha antibody |
Protocols for AP2 alpha antibody |
Price and availability of products related to AP2 alpha antibody |
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AP2 alpha antibody - related products:
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AP2 alpha antibody - other tools:
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Contact Abcam with a Technical Enquiry about AP2 alpha antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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