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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-AP2 alpha antibody
See all AP2 alpha products (6) ...
Description
Rabbit polyclonal to AP2 alpha
Tested applications
WB, IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Chicken
Immunogen
Synthetic peptide: SHTDNNAKSSDKEEKHRK conjugated to KLH, corresponding to amino acids 420-437 of Human AP2 alpha.
SHTDNNAKSS DKEEKHRK
Positive control
Nuclear extract of HeLa human epithelioid carcinoma cells (western blotting) and of fixed cultured HeLa cells (immunofluorescence).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Immunocytochemistry/ Immunofluorescence-AP2 alpha antibody(ab11828)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab11828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
IF
IF: 1/100IF: 1/100
Target
Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.
Involvement in disease
Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.
Sequence similarities
Belongs to the AP-2 family.
Domain
The WW-binding motif mediates interaction with WWOX.
Post-translational
modifications
Sumoylated on Lys-10; which inhibits transcriptional activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP05549
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
see all
Database links
- Entrez Gene: 7020 Human
- Entrez Gene: 21418 Mouse
- Entrez Gene: 306862 Rat
- Omim: 107580 Human
- SwissProt: P05549 Human
- SwissProt: P34056 Mouse
- SwissProt: P58197 Rat
- SwissProt: Q9N0N3 Sheep
see all
Anti-AP2 alpha antibody images:
Immunocytochemistry/ Immunofluorescence-AP2 alpha antibody(ab11828)
ICC/IF image of ab11828 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11828, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-AP2 alpha antibody (ab11828)
This product has been referenced in:
- Aihara Yet al. Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture. Int J Dev Biol 54:1287-94 (2010). ICC/IF; Mouse.Read more (PubMed: 20711997) »
See 1 publication for this product
Publishing research using ab11828? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"