|
|
|
|
Western blot - AP2 alpha antibody (ab52222)
(enlarge)
|
|
Electrophoretic Mobility Shift Assay - AP2 alpha antibody (ab52222)
(enlarge)
|
|
|
|
|
Product Name
|
AP2 alpha antibody - ChIP Grade
|
| |
See all AP2 alpha - transcription factor antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to AP2 alpha - ChIP Grade
|
|
Immunogen
|
Synthetic peptide derived from human AP2 alpha.
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Specificity
|
ab52222 detects endogenous levels of total AP2 alpha protein.
|
|
Tested applications
(see key)
|
ChIP, ELISA, EMSA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 19884909).
EMSA: Use at an assay dependent dilution.
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Extracts from COLO205 cells
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Other factors
|
|
Relevance
|
Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AP2 alpha antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for AP2 alpha antibody - ChIP Grade images, references, products related to ab52222 and other tools.
|
| |
AP2 alpha antibody - ChIP Grade images:
|
 Western blot - AP2 alpha antibody (ab52222)
All lanes : AP2 alpha antibody - ChIP Grade (ab52222) at 1/500 dilution
Lane 1 : extracts from COLO205 cells,
Lane 2 : extracts from COLO205 cells, with immunizing peptide
Predicted band size : 48 kDa
Observed band size : 48 kDa
|
| |
Electrophoretic Mobility Shift Assay - AP2 alpha antibody (ab52222)
Antibody anti-AP-2 alpha (ab52222) was used in an Electrophoretic Mobility Shift Assay (EMSA) to supershift the protein-DNA complex.
Radiolabelled, double-stranded DNA oligonucleotides (10.000 cpm per lane) harbouring a binding site for AP-2 alpha were incubated with each 2 µg of nuclear extract (NE) from HeLa and Caski cells, respectively. Samples were incubated for 30 minutes at room temperature to allow the formation of protein-DNA complexes. 2 µg of anti-AP-2 alpha antibody were added to the samples (as indicated) and incubated for further 60 minutes at 4°C. Samples were separated in a 5.5% PAGE for 30 minutes at 280 V and further 75 minutes at 350 V. The Gel was dried under vacuum and for autoradiography a X-ray film was exposed with an intensifying screen for 2 days at -80°C.
Specific protein-DNA complexes were quantitatively supershifted with antibody anti-AP-2 alpha (ab52222), verifying the binding of AP-2 alpha to the DNA oligonucleotide.
Rating: *****
This image was kindly provided by Dr Lars Krueger.
|
| |
References for AP2 alpha antibody - ChIP Grade (ab52222)
|
|
This product has been used in:
Kolev MV et al. Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration. Pharmacogenomics J 10:12-9 (2010). ChIP; Human. PubMed: 19884909
If you publish research using ab52222 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to AP2 alpha
|
| |
AP2 alpha antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding AP2 alpha antibody - ChIP Grade |
Customer FAQs regarding AP2 alpha antibody - ChIP Grade |
Protocols for AP2 alpha antibody - ChIP Grade |
Price and availability of products related to AP2 alpha antibody - ChIP Grade |
| |
AP2 alpha antibody - ChIP Grade - related products:
|
|
|
| |
AP2 alpha antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about AP2 alpha antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
