AP2 alpha protein (Tagged-His Tag) (ab91893)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- 100 kDa coated vesicle protein AAdapter-related protein complex 2 alpha-1 subunitAdaptin alpha
- Adaptin alpha AAdaptor protein complex AP 2 alpha 1 subunitadaptor protein complex AP 2 subunit alpha 1Adaptor protein complex AP-2 subunit alpha-1Adaptor protein complex AP2 alpha 1 subunitadaptor protein complex AP2 subunit alpha 1Adaptor related protein complex 2 alpha 1 subunitAdaptor related protein complex AP 2 alpha 1 subunitAdaptor related protein complex AP2 alpha 1 subunitADTAAAlpha A adaptinAlpha adaptin AAlpha-adaptin Aalpha1 adaptinAlpha1-adaptinAP 2 alphaAP 2 complex subunit alpha 1AP-2 complex subunit alpha-1AP2 complex subunit alpha 1AP2-ALPHAAp2a1AP2A1_HUMANCLAPA 1CLAPA1Clathrin adaptor complex AP2. alpha subunitClathrin assembly protein complex 2 alpha A large chainClathrin assembly protein complex 2 alpha-A large chainClathrin associated / assembly / adaptor protein large alpha 1clathrin associated assembly adaptor protein large alpha 1clathrin associated/assembly/adaptor protein, large, alpha 1Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunitPlasma membrane adaptor HA2/AP2 adaptin alpha A subunit
AP2 alpha protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for AP2 alpha protein (Tagged-His Tag) (ab91893)
ab91893 has not yet been referenced specifically in any publications.