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Western blot - AP2M1 antibody [EP2695Y] (ab75995)
![Western blot - AP2M1 antibody [EP2695Y] (ab75995)](/ps/datasheet/images/ab75995_1.JPG)
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Flow Cytometry - AP2M1 antibody [EP2695Y] (ab75995)
![Flow Cytometry - AP2M1 antibody [EP2695Y] (ab75995)](/ps/datasheet/images/ab75995_2.JPG)
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Product Name
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AP2M1 antibody [EP2695Y]
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See all AP2M1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP2695Y] to AP2M1
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Immunogen
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A synthetic peptide corresponding to residues near the C terminus of human AP2M1
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
WB: 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
Is unsuitable for IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF-7 and SKBR-3 cell lysates
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Cellular localization
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Plasma membrane
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
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Relevance
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AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. It is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.
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AP2M1 antibody [EP2695Y] (ab75995) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP2695Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AP2M1 antibody [EP2695Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AP2M1 antibody [EP2695Y] images, references, products related to ab75995 and other tools.
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AP2M1 antibody [EP2695Y] images:
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 Western blot - AP2M1 antibody [EP2695Y] (ab75995)
All lanes : AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
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Flow Cytometry - AP2M1 antibody [EP2695Y] (ab75995)
Flow cytometric analysis of AP2M1 in permeabilized MCF-7 cells using ab75995 (red) at 1/60 dilution or a rabbit IgG antibody (negative) (green).
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Search PubMed (MEDLINE) for references to AP2M1
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AP2M1 antibody [EP2695Y] - more information
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Customer reviews (feedback) regarding AP2M1 antibody [EP2695Y] |
Customer FAQs regarding AP2M1 antibody [EP2695Y] |
Protocols for AP2M1 antibody [EP2695Y] |
Price and availability of products related to AP2M1 antibody [EP2695Y] |
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AP2M1 antibody [EP2695Y] - related products:
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AP2M1 antibody [EP2695Y] - other tools:
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Contact Abcam with a Technical Enquiry about AP2M1 antibody [EP2695Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - AP2M1 antibody [EP2695Y] (ab75995)](/index.html?datasheet=75995#ab75995_1.JPG)
![Flow Cytometry - AP2M1 antibody [EP2695Y] (ab75995)](/index.html?datasheet=75995#ab75995_2.JPG)
