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Read our guarantee »Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Anti-AP2M1 antibody [EP2695Y]
See all AP2M1 products (7) ...
Rabbit monoclonal [EP2695Y] to AP2M1
WB, ICC, Flow Cytmore details
Reacts with
Mouse, Rat, Human
A synthetic peptide corresponding to residues near the C terminus of human AP2M1
MCF-7 and SKBR-3 cell lysates
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Tissue culture supernatant
Monoclonal
EP2695Y
IgG
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Our Abpromise guarantee covers the use of ab75995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 50 kDa.
ICC: 1/100 - 1/250.
Flow Cyt: 1/60
Is unsuitable for ,IHC-P or IP.
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain.
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Target information above from: UniProt accessionQ96CW1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - AP2M1 antibody [EP2695Y] (ab75995)
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Flow Cytometry - AP2M1 antibody [EP2695Y] (ab75995)
Flow cytometric analysis of AP2M1 in permeabilized MCF-7 cells using ab75995 (red) at 1/60 dilution or a rabbit IgG antibody (negative) (green).
This product has been referenced in:
See 1 publication for this product
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All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Flow cytometric analysis of AP2M1 in permeabilized MCF-7 cells using ab75995 (red) at 1/60 dilution or a rabbit IgG antibody (negative) (green).
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