Anti-AP2S1 antibody (ab54661)

Overview

• Product nameAnti-AP2S1 antibodySee all AP2S1 primary antibodies ...
• Description
  Mouse monoclonal to AP2S1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Recombinant Fragment
  Predicted to work with: Human
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-143 of Human AP2S1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Adapters

Applications

Target

• FunctionComponent of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
• Sequence similaritiesBelongs to the adaptor complexes small subunit family.
• Cellular localizationCell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Target information above from: UniProt accession P53680 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1175 Human
  • Omim: 602242 Human
  • SwissProt: P53680 Human
  • Unigene: 119591 Human

• Alternative names
  Adapter-related protein complex 2 sigma subunit antibodyAdaptor protein complex AP 2 subunit sigma antibodyAdaptor protein complex AP-2 subunit sigma antibody

  Adaptor protein complex AP2 subunit sigma antibodyAdaptor related protein complex 2 sigma 1 subunit antibodyAI043088 antibodyAP 17 antibodyAP 2 complex subunit sigma 1 antibodyAP-2 complex subunit sigma antibodyAP17 antibodyAP17 delta antibodyAP2 complex subunit sigma 1 antibodyAp2s1 antibodyAP2S1_HUMAN antibodyCLAPS 2 antibodyCLAPS2 antibodyClathrin adaptor complex AP2, sigma subunit antibodyClathrin adaptor protein AP17 antibodyClathrin assembly protein 2 small chain antibodyClathrin associated/assembly/adaptor protein small 2 antibodyClathrin associated/assembly/adaptor protein small 2, 17-KD antibodyClathrin associated/assembly/adaptor protein, small 2 (17kD) antibodyClathrin coat assembly protein AP17 antibodyClathrin coat associated protein AP17 antibodyClathrin coat-associated protein AP17 antibodyHA2 17 kDa subunit antibodyMGC62945 antibodyPlasma membrane adaptor AP 2 17 kDa protein antibodyPlasma membrane adaptor AP-2 17 kDa protein antibodyPlasma membrane adaptor AP2 17 kDa protein antibodySigma adaptin 3b antibodySigma2 adaptin antibodySigma2-adaptin antibody

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Anti-AP2S1 antibody images

• 

  Western blot - AP2S1 antibody (ab54661)
  Western blot against tagged recombinant protein immunogen using ab54661 AP2S1 antibody at 1ug/ml. Predicted band size of immunogen is 42 kDa.

  This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.