Anti-AP3B2 antibody (ab113100)
Key features and details
- Rabbit polyclonal to AP3B2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-AP3B2 antibody
See all AP3B2 primary antibodies -
Description
Rabbit polyclonal to AP3B2 -
Host species
Rabbit -
Specificity
At least three isoforms of AP3B2 are known to exist; this antibody will recognize the two larger isoforms. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
An 18 amino acid synthetic peptide from near the amino terminus of Human AP3B2 (NP_004635).
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Positive control
- WB: Rat brain tissue lysate, mouse brain lysate, IHC-P: Mouse brain tissue. ICC: Mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab113100 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 119 kDa.
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 119 kDa. |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. -
Tissue specificity
Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). -
Sequence similarities
Belongs to the adaptor complexes large subunit family. -
Developmental stage
Expressed from early in development through adulthood. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 11775 Mouse
- Entrez Gene: 308777 Rat
- SwissProt: Q9JME5 Mouse
- Unigene: 322894 Mouse
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Alternative names
- Adapter-related protein complex 3 subunit beta-2 antibody
- Adaptor protein complex AP-3 subunit beta-2 antibody
- Adaptor related protein complex 3 beta 2 subunit antibody
see all
Images
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Western Blot of rat brain tissue lysate labeling AP3B2 with Anti-AP3B2 antibody (ab113100) at (1) 1 and (2) 2µg/ml.
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Immunofluorescence of AP3B2 in mouse brain tissue with ab113100 at 20 ug/mL.
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Lane 1 : Anti-AP3B2 antibody (ab113100) at 1 µg/ml
Lane 2 : Anti-AP3B2 antibody (ab113100) at 2 µg/ml
All lanes : Mouse brain lysate
Predicted band size: 119 kDa -
Lane 1 : Anti-AP3B2 antibody (ab113100) at 1 µg/ml
Lane 2 : Anti-AP3B2 antibody (ab113100) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 119 kDa -
Immunohistochemical analysis of mouse brain tissue, staining AP3B2 with ab113100 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113100 has not yet been referenced specifically in any publications.