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Anti-APAF1 antibody [5E11] (ab36824)

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Overview

Product name

Anti-APAF1 antibody [5E11]
See all APAF1 products (12) ...

Description

Mouse monoclonal [5E11] to APAF1

Tested applications

IHC (PFA fixed)more details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to amino acids near the carboxy terminus of human APAF1.

Positive control

K562 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

5E11

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial

  • Immunohistochemistry - APAF1 antibody [5E11] (ab36824)Immunohistochemistry - APAF1 antibody [5E11] (ab36824) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC (PFA fixed)

     

Application notes

Immunohistochemistry (PFA fixed): Use at a concentration of 0.5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Tissue specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Sequence similarities

Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.

Domain

The CARD domain mediates interaction with APIP.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionO14727 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • APAF 1 antibody
  • Apaf-1 antibody
  • APAF_HUMAN antibody
  • Apaf1 antibody
  • Apoptotic peptidase activating factor 1 antibody
  • Apoptotic protease activating factor 1 antibody
  • Apoptotic protease activating factor antibody
  • Apoptotic protease-activating factor 1 antibody
  • CED 4 antibody
  • CED4 antibody
  • KIAA0413 antibody
see all

Database links

see all

Anti-APAF1 antibody [5E11] images:

  Immunohistochemistry - APAF1 antibody [5E11] (ab36824)

Immunohistochemistry - APAF1 antibody [5E11] (ab36824)

ab36824 at 0.5µg/ml staining APAF1 in K562 cells by IHC

References for Anti-APAF1 antibody [5E11] (ab36824)

ab36824 has not yet been referenced specifically in any publications.

Publishing research using ab36824? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"