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Anti-APAF1 antibody [E38] (ab32372) at 1/500 dilution + HeLa cell lysate
Predicted band size : 141 kDa
Observed band size : 135 kDa (why is the actual band size different from the predicted?)
Ab32372, at a dilution of 1/50, staining APAF1 in paraffin embedded stomach adenocarcinoma by Immunohistochemistry.
ab32372 staining APAF1 in human leukocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.3% Triton X-100, blocked with 2% serum for 2 hours at 25°C, and then incubated with ab32372 at a 1/250 dilution for 9 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
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