You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-APAF1 antibody [E38] (ab32372)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-APAF1 antibody [E38]
See all APAF1 products (12) ...

Description

Rabbit monoclonal [E38] to APAF1

Tested applications

ICC/IF, WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues near N terminus of human Apaf1

Positive control

WB: HeLa cell lysate. IHC-P: Stomach adenocarcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E38

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial

  • Western blot - APAF1 antibody [E38] (ab32372)Western blot - APAF1 antibody [E38] (ab32372) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - APAF1 antibody [E38] (ab32372)Immunohistochemistry (Paraffin-embedded sections) - APAF1 antibody [E38] (ab32372) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.

WB: 1/500. Detects a band of approximately 135 kDa (predicted molecular weight: 141 kDa).

Is unsuitable for FACS or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Tissue specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Sequence similarities

Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.

Domain

The CARD domain mediates interaction with APIP.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionO14727 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • APAF 1 antibody
  • Apaf-1 antibody
  • APAF_HUMAN antibody
  • Apaf1 antibody
  • Apoptotic peptidase activating factor 1 antibody
  • Apoptotic protease activating factor 1 antibody
  • Apoptotic protease activating factor antibody
  • Apoptotic protease-activating factor 1 antibody
  • CED 4 antibody
  • CED4 antibody
  • KIAA0413 antibody
see all

Database links

Anti-APAF1 antibody [E38] images:

  Western blot - APAF1 antibody [E38] (ab32372)

Western blot - APAF1 antibody [E38] (ab32372)

Anti-APAF1 antibody [E38] (ab32372) at 1/500 dilution + HeLa cell lysate

Predicted band size : 141 kDa
Observed band size : 135 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - APAF1 antibody [E38] (ab32372)

Immunohistochemistry (Paraffin-embedded sections) - APAF1 antibody [E38] (ab32372)

Ab32372, at a dilution of 1/50, staining APAF1 in paraffin embedded stomach adenocarcinoma by Immunohistochemistry.

References for Anti-APAF1 antibody [E38] (ab32372)

ab32372 has not yet been referenced specifically in any publications.

Publishing research using ab32372? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"