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Anti-APAF1 antibody (ab14228)

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Overview

Product name

Anti-APAF1 antibody
See all APAF1 products (12) ...

Description

Chicken polyclonal to APAF1

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide: PEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQ NKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDL SI, corresponding to amino acids 307-388 of Human APAF1.

PEQAHSIIKE CKGSPLVVSL IGALLRDFPN RWEYYLKQLQ NKQFKRIRKS SSYDYEALDE AMSISVEMLR EDIKDYYTDL SI

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgY

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial

  • Western blot - APAF1 antibody (ab14228)Western blot - APAF1 antibody (ab14228) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab14228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Tissue specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Sequence similarities

Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.

Domain

The CARD domain mediates interaction with APIP.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionO14727 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • APAF 1 antibody
  • Apaf-1 antibody
  • APAF_HUMAN antibody
  • Apaf1 antibody
  • Apoptotic peptidase activating factor 1 antibody
  • Apoptotic protease activating factor 1 antibody
  • Apoptotic protease activating factor antibody
  • Apoptotic protease-activating factor 1 antibody
  • CED 4 antibody
  • CED4 antibody
  • KIAA0413 antibody
see all

Database links

see all

Anti-APAF1 antibody images:

  Western blot - APAF1 antibody (ab14228)

Western blot - APAF1 antibody (ab14228)



Predicted band size : 139 kDa


Western blot analysis of APAF1 with an E coli-derived fusion protein as test antigen, using ab14228 diluted 1/2000.

References for Anti-APAF1 antibody (ab14228)

ab14228 has not yet been referenced specifically in any publications.

Publishing research using ab14228? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"