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Western blot - APAF1 antibody (ab14228)
(enlarge)
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Product Name
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APAF1 antibody
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See all APAF1 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to APAF1
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Immunogen
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Synthetic peptide: PEQAHSIIKE CKGSPLVVSL IGALLRDFPN RWEYYLKQLQ NKQFKRIRKS SSYDYEALDE AMSISVEMLR EDIKDYYTDL corresponding to amino acids 307-388 of Human APAF1.
BLAST 'PEQAHSIIKE CKGSPLVVSL IGALLRDFPN RWEYYLKQLQ NKQFKRIRKS SSYDYEALDE AMSISVEMLR EDIKDYYTDL ' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
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Rat
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500. Predicted molecular weight: 139 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial
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Relevance
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homolog of the key cell death gene CED 4 in C. elegans was identified recently from human and mouse and designated APAF1 for Apoptosis protease activating factor 1. APAF1 binds to Cytochrome c (APAF2) and Caspase 9 (APAF3), which leads to Caspase 9 activation. Activated Caspase 9 in turn cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 can also associate with Caspase 4 and Caspase 8. APAF1 transcript is ubiquitously expressed in human tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APAF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APAF1 antibody images, references, products related to ab14228 and other tools.
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APAF1 antibody images:
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 Western blot - APAF1 antibody (ab14228)
Predicted band size : 139 kDa
Western blot analysis of APAF1 with an E coli-derived fusion protein as test antigen, using ab14228 diluted 1/2000.
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Search PubMed (MEDLINE) for references to APAF1
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APAF1 antibody - more information
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Customer reviews (feedback) regarding APAF1 antibody |
Customer FAQs regarding APAF1 antibody |
Protocols for APAF1 antibody |
Price and availability of products related to APAF1 antibody |
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APAF1 antibody - related products:
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APAF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about APAF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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