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Western blot - APAF1 antibody (ab14228)
Western blot - APAF1 antibody (ab14228)
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Product Name 

APAF1 antibody

 

See all APAF1 antibodies (11)...

Product type 

Primary antibodies

Description 

Chicken polyclonal to APAF1

Immunogen 

Synthetic peptide: PEQAHSIIKE CKGSPLVVSL IGALLRDFPN RWEYYLKQLQ NKQFKRIRKS SSYDYEALDE AMSISVEMLR EDIKDYYTDL corresponding to amino acids 307-388 of Human APAF1.
BLAST 'PEQAHSIIKE CKGSPLVVSL IGALLRDFPN RWEYYLKQLQ NKQFKRIRKS SSYDYEALDE AMSISVEMLR EDIKDYYTDL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500. Predicted molecular weight: 139 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial

Relevance 

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homolog of the key cell death gene CED 4 in C. elegans was identified recently from human and mouse and designated APAF1 for Apoptosis protease activating factor 1. APAF1 binds to Cytochrome c (APAF2) and Caspase 9 (APAF3), which leads to Caspase 9 activation. Activated Caspase 9 in turn cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 can also associate with Caspase 4 and Caspase 8. APAF1 transcript is ubiquitously expressed in human tissues.

 

Alternative names APAF1 antibody (ab14228)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

317    (Human)
11783    (Mouse)
78963    (Rat)

GeneCard

    

GC12P097541    (Human)

Omim

    

602233    (Human)

SwissProt

    

O14727    (Human)
O88879    (Mouse)
Q9EPV5    (Rat)

Unigene

    

373575    (Human)
220289    (Mouse)
64522    (Rat)

Raised in 

Chicken

Clonality 

Polyclonal

Isotype 

IgY

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APAF1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for APAF1 antibody images, references, products related to ab14228 and other tools.

 

APAF1 antibody images:

Product image  Western blot - APAF1 antibody (ab14228)

Western blot - APAF1 antibody (ab14228)



Predicted band size : 139 kDa


Western blot analysis of APAF1 with an E coli-derived fusion protein as test antigen, using ab14228 diluted 1/2000.

 
 

Search PubMed (MEDLINE) for references to APAF1

 

APAF1 antibody - more information

Customer reviews (feedback) regarding APAF1 antibody

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APAF1 antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for APAF1 antibody

ab6749

  

FITC antibody

ab6751

  

Texas Red ® antibody

ab6752

  

Biotin antibody

ab6753

  

HRP antibody

ab6874

  

Rhodamine antibody

ab6878

  

Alkaline Phosphatase antibody

ab46969

  

FITC antibody

 

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