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Products:Cell Biology >> Apoptosis >> Mitochondrial
Overview
Product name
Anti-APAF1 antibody
See all APAF1 products (12) ...
Description
Rabbit polyclonal to APAF1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 1158-1177 of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid
Epitope
TFYTNGTNLKKIHVSPDFKT
Positive control
Human heart tissue lysate
General notes
Apoptosis Protease Activating Factor
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 20 µg/ml
WB: 1/500 - 1/2000.Predicted molecular weight: 130 kDa.
Target
Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Tissue specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Sequence similarities
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
Domain
The CARD domain mediates interaction with APIP.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO14727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
Database links
- Entrez Gene: 317 Human
- Entrez Gene: 11783 Mouse
- Entrez Gene: 78963 Rat
- Omim: 602233 Human
- SwissProt: O14727 Human
- SwissProt: O88879 Mouse
- SwissProt: Q9EPV5 Rat
- Unigene: 728891 Human
see all
Anti-APAF1 antibody images:
Western blot - APAF1 antibody (ab2000)
All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml
Lane 1 : Human heart tissue lysate with absence of blocking peptide
Lane 2 : Human heart tissue lysate with presence of blocking peptide
Predicted band size : 130 kDa
Observed band size : 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APAF1 antibody (ab2000)
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
References for Anti-APAF1 antibody (ab2000)
ab2000 has not yet been referenced specifically in any publications.
Publishing research using ab2000? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"