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Western blot - APAF1 antibody (ab2000)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APAF1 antibody (ab2000)
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Product Name
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APAF1 antibody
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See all APAF1 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to APAF1
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Immunogen
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Synthetic peptide, corresponding to amino acids 1158-1177 of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid
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Reacts with
(species key)
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Hu, Ms, Rat
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Epitope
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TFYTNGTNLKKIHVSPDFKT
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 20 µg/ml.
WB: 1/500 - 1/2000. Predicted molecular weight: 130 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human heart tissue lysate
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial
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Relevance
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homolog of the key cell death gene CED 4 in C. elegans was identified recently from human and mouse and designated APAF1 for Apoptosis protease activating factor 1. APAF1 binds to Cytochrome c (APAF2) and Caspase 9 (APAF3), which leads to Caspase 9 activation. Activated Caspase 9 in turn cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 can also associate with Caspase 4 and Caspase 8. APAF1 transcript is ubiquitously expressed in human tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Apoptosis Protease Activating Factor
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APAF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APAF1 antibody images, references, products related to ab2000 and other tools.
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APAF1 antibody images:
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 Western blot - APAF1 antibody (ab2000)
All lanes : APAF1 antibody (ab2000) at 1 µg/ml
Lane 1 : Human heart tissue lysate with absence of blocking peptide
Lane 2 : Human heart tissue lysate with presence of blocking peptide
Predicted band size : 130 kDa
Observed band size : 130 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APAF1 antibody (ab2000)
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
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Search PubMed (MEDLINE) for references to APAF1
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APAF1 antibody - more information
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Customer reviews (feedback) regarding APAF1 antibody |
Customer FAQs regarding APAF1 antibody |
Protocols for APAF1 antibody |
Price and availability of products related to APAF1 antibody |
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APAF1 antibody - related products:
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APAF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about APAF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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