APAF1 antibody (ab2001)

Product Name 

APAF1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to APAF1

Immunogen 

Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1. The sequence of theimmunogenic peptide differs from that of murine Apaf1 byone amino acid.

Reacts with 
(species key)

Hu, Ms, Rat

Epitope 

HREALEKDIKTSYIMDHC

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/2000. Detects a band of approximately 141 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa whole cell lysate.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial

Relevance 

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homolog of the key cell death gene CED 4 in C. elegans was identified recently from human and mouse and designated APAF1 for Apoptosis protease activating factor 1. APAF1 binds to Cytochrome c (APAF2) and Caspase 9 (APAF3), which leads to Caspase 9 activation. Activated Caspase 9 in turn cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 can also associate with Caspase 4 and Caspase 8. APAF1 transcript is ubiquitously expressed in human tissues.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Apoptosis Protease Activating Factor.

See below for APAF1 antibody images, references, products related to ab2001 and other tools.

APAF1 antibody images:

References for APAF1 antibody (ab2001)

  This product has been used in: (two most recent references)

Cobbold LC et al. Identification of internal ribosome entry segment (IRES)-trans-acting factors for the Myc family of IRESs. Mol Cell Biol 28:40-9 (2008). WB. PubMed: 17967896

Potokar M et al. Subcellular localization of Apaf-1 in apoptotic rat pituitary cells. Am J Physiol Cell Physiol 290:C672-7 (2006). PubMed: 16207793

See all 3 publication references for this product.


If you publish research using ab2001 please let us know so that we can cite the reference on this datasheet.

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