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Products:Cell Biology >> Apoptosis >> Mitochondrial
Overview
Product name
Anti-APAF1 antibody
See all APAF1 products (12) ...
Description
Rabbit polyclonal to APAF1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1. The sequence of theimmunogenic peptide differs from that of murine Apaf1 byone amino acid.
Epitope
HREALEKDIKTSYIMDHC
Positive control
HeLa whole cell lysate.
General notes
Apoptosis Protease Activating Factor.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml
WB: 1/1000 - 1/2000.Detects a band of approximately 141 kDa.
Target
Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Tissue specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Sequence similarities
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
Domain
The CARD domain mediates interaction with APIP.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO14727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
Database links
- Entrez Gene: 317 Human
- Entrez Gene: 11783 Mouse
- Entrez Gene: 78963 Rat
- Omim: 602233 Human
- SwissProt: O14727 Human
- SwissProt: O88879 Mouse
- SwissProt: Q9EPV5 Rat
- Unigene: 728891 Human
see all
Anti-APAF1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APAF1 antibody (ab2001)
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC
Western blot - APAF1 antibody (ab2001)
All lanes : Anti-APAF1 antibody (ab2001) at 1 µg/ml
Lane 1 : K562 cell lysate in absence of blocking peptide
Lane 2 : K562 cell lysate in presence of blocking peptide.
Observed band size : 141 kDa (why is the actual band size different from the predicted?)
References for Anti-APAF1 antibody (ab2001)
This product has been referenced in:
- Cobbold LCet al. Identification of internal ribosome entry segment (IRES)-trans-acting factors for the Myc family of IRESs. Mol Cell Biol 28:40-9 (2008). WB.Read more (PubMed: 17967896) »
- Potokar Met al. Subcellular localization of Apaf-1 in apoptotic rat pituitary cells. Am J Physiol Cell Physiol 290:C672-7 (2006).Read more (PubMed: 16207793) »
See all 3 publications for this product
Publishing research using ab2001? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"