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Products:Cell Biology >> Apoptosis >> Mitochondrial
Overview
Product name
Anti-APAF1 antibody, prediluted
See all APAF1 products (12) ...
Description
Rabbit polyclonal to APAF1, prediluted
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide (Human) (C terminal).
Positive control
Tested with testis cells.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cell Biology >> Apoptosis >> Mitochondrial
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- APAF1 antibody, prediluted (ab15178)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Application notes
IHC-P: Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Tissue specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Sequence similarities
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
Domain
The CARD domain mediates interaction with APIP.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO14727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
Database links
- Entrez Gene: 317 Human
- Entrez Gene: 11783 Mouse
- Entrez Gene: 78963 Rat
- Omim: 602233 Human
- SwissProt: O14727 Human
- SwissProt: O88879 Mouse
- SwissProt: Q9EPV5 Rat
- Unigene: 728891 Human
see all
Anti-APAF1 antibody, prediluted images:
References for Anti-APAF1 antibody, prediluted (ab15178)
ab15178 has not yet been referenced specifically in any publications.
Publishing research using ab15178? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"