Recombinant Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15941] to Mint-1 - N-terminal
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Mint-1 antibody [EPR15941] - N-terminal
See all Mint-1 primary antibodies -
Description
Rabbit monoclonal [EPR15941] to Mint-1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, Human cerebellum, Human Transitional cell carcinoma of bladder.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15941 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 156 kDa (predicted molecular weight: 93 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/40.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 156 kDa (predicted molecular weight: 93 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40. |
Target
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Function
Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. -
Tissue specificity
Brain and spinal cord. -
Sequence similarities
Contains 2 PDZ (DHR) domains.
Contains 1 PID domain. -
Domain
Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 320 Human
- Omim: 602414 Human
- SwissProt: Q02410 Human
- Unigene: 171939 Human
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Alternative names
- Adapter protein X11 alpha antibody
- Adapter protein X11alpha antibody
- Amyloid beta A4 precursor protein-binding family A member 1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human transitional cell carcinoma of the bladder labeling Mint-1 with ab190359 at 1/50 dilution and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/50000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 93 kDa
Observed band size: 156 kDa why is the actual band size different from the predicted? -
Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/10000 dilution + Human cerebellum tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 93 kDa
Observed band size: 156 kDa why is the actual band size different from the predicted? -
Immunoprecipitation of Human fetal brain labeling Mint-1 with ab190359 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab190359 has not yet been referenced specifically in any publications.