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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-APC antibody [ALi 12-28]
See all APC products (11) ...
Description
Mouse monoclonal [ALi 12-28] to APC
Specificity
This antibody recognises the N-terminal region of the APC protein.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein.
Epitope
ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
ALi 12-28
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts.)
IHC-Fr: Use at an assay dependent dilution.
Target
Relevance
Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Cellular localization
Cell junction; adherens junction
Alternative names
- Adenomatous Polyposis Coli antibody
- APC antibody
- CC1 antibody
see all
Database links
- Entrez Gene: 324 Human
- Omim: 175100 Human
- SwissProt: P25054 Human
References for Anti-APC antibody [ALi 12-28] (ab58)
This product has been referenced in:
- Tanneberger Ket al. Structural and functional characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1). J Biol Chem 286:19204-14 (2011). WB; Human.Read more (PubMed: 21498506) »
- Schneikert Jet al. APC mutations in colorectal tumours from FAP patients are selected for CtBP-mediated oligomerization of truncated APC. Hum Mol Genet 20:3554-64 (2011).Read more (PubMed: 21665989) »
See all 9 publications for this product
Publishing research using ab58? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"