APC antibody [ALi 12-28] (ab58)

Product Name 

APC antibody [ALi 12-28]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ALi 12-28] to APC

Immunogen 

Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein.

Reacts with 

Hu

Does not react with 
(species key)

Ms

Specificity 

This antibody recognises the N-terminal region of the APC protein.

Epitope 

ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.).

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts.

Cellular localization 

Cell junction; adherens junction

Research areas 

Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

Relevance 

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration.
Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ALi 12-28

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

Batch dependent within range: 0.95 - 0.95 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for APC antibody [ALi 12-28] (ab58)

  This product has been used in: (two most recent references)

Arnold HK et al. The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc. EMBO J 28:500-12 (2009). WB; Human. PubMed: 19131971

Kouzmenko AP et al. Ligand-dependent interaction between estrogen receptor alpha and adenomatous polyposis coli. Genes Cells 13:723-30 (2008). PubMed: 18498351

See all 6 publication references for this product.


If you publish research using ab58 please let us know so that we can cite the reference on this datasheet.

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