Anti-APC antibody [CC-1] (ab16794)

No staining with ab16794 in mouse spinal cord sections.

ANSWER:

Thank you for your call today and for letting us know about the trouble with ab16794.

As we discussed, I'm sending a free of charge vial of ab15270 on the order ***, which should arrive in the next couple of days.

Please keep me updated about how this replacement antibody works in your staining, and let me know if there is anything else that we can do for you.

Dear I'm looking for a anti APC antibody that could recognize the N-terminus of the APC gene in mouse. I found in your website the ab16794 antibody but it has been made in mouse. I want to ask you if there is any possibility to have an alquot to test this antibody in IHC on murine intestinal tissue...or if not, you can give me some advice to do the IHC on paraffin and frozen section. Thanks a lot

ANSWER:

Thank you for contacting us.

We recommend doing extra steps while performing mouse on mouse staining. Please check the recommended guidelines given in below attached document.

http://www.abcam.com/index.html?pageconfig=resource&rid=11465

We also have other antibodies in catalogue that are raised in Rabbit. They are suitable for IHC-P with mouse tissues; you can choose one of these if there is not special of choosing mouse antibody only.

We unfortunately do not provide free samples for testing purpose. All of the products come with Abcam Abpromise guarantee; which states the money will be refunded in case the products do not work in applications and species as stated on the datasheet. 

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Can you please send me the protocol for using ab16794 in IHC-P?

ANSWER:

Thank you for contacting us.

Please find the protocol used to test this antibody in IHC-Fr and ICC attached. This antibody has been used successfully in IHC-P on rat brain sections in the reference PMID 21056562. In this reference, heat mediated antigen retrieval was performed using an EDTA buffer at pH 9.0.

I hope this helps, please let me know if you need any additional information.

I have a customer enquiring about product ab16794, please refer below:

I would like to get further information about APC antibody [CC-1]; Catalogue number: (ab16794). I am planning to use it for detecting oligodendrocytes in the mouse corpus callosum on paraffin embedded sections. I already had a look at the datasheet on internet and it is said that this antibody works in paraffin sections. Do I need to perform antigen retrieval? If so, what kind of antigen retrieval is recommended? I am also concerned about the specificity of the antibody. Although, in a revision of the available literature, CC-1 Ab is extensively employed for exclusively detecting oligodendrocytes (even in complex samples where there are also astrocytes), it seems that binds to both oligodendrocytes and astrocytes according with the electronic datasheet. At what extent CC-1 binds to astrocytes? I have used GFAP Ab (which detects astrocytes) on my samples, and I have observed some positive cells; is this going to represent a problem? By this I mean, is it possible to use CC-1 Ab in a complex sample, where astrocytes and oligodendrocytes are present, for detecting oligodendrocytes? Also, would you be so kind to send me more sample pictures of CC-1 positive cells? Please let me know if you have any suggestions.

ANSWER:

Unfortunately we do not have a lot more information available from the datasheet from the source of ab16794.

Regarding the antigen retrieval method, as the legend of the image states sections were pretreated with heat using a pressure cooker prior to staining.

Regarding the specificity of this antibody, indeed it will stain both oligodendrocytes and astrocytes but we do not have information regarding the extent of the binding to astrocytes so GFAP co-staining may prove problematic. We unfortunately do not have images for ICC but the image on the datasheet shows an IHC-P staining.

Sorry I couldn't help you more on this occasion,