Anti-APC antibody [CC-1] (ab16794)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APC antibody [CC-1] (ab16794)

IHC-F formalin-fixed, paraffin embedded human cerebellum with ab16794.

Antibody was used at a concentration of 2.5 mg/ml and sections were pretreated with heat using a pressure cooker prior to staining. Detection with DAB and hematoxylin as a counterstain

  Immunocytochemistry/ Immunofluorescence - APC antibody [CC-1] (ab16794)

ICC/IF image of ab16794 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16794, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.