APC antibody [CC-1] (ab16794)

Product Name 

APC antibody [CC-1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [CC-1] to APC

Immunogen 

Immunogen used was a recombinant amino terminal fragment of APC

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody has been shown to detect oligodendrocytes and astrocytes.

Epitope 

Epitope is within amino acid residues 1 - 226 of APC.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr: Use at a concentration of 5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

Relevance 

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration.
Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

CC-1

Isotype 

IgG2b

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for APC antibody [CC-1] images, references, products related to ab16794 and other tools.

APC antibody [CC-1] images:

References for APC antibody [CC-1] (ab16794)

  This product has been used in: (two most recent references)

Almad A & McTigue DM Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord. J Comp Neurol 518:785-99 (2010). IHC-FoFr; Rat. PubMed: 20058304

Zhang Y et al. Notch1 signaling plays a role in regulating precursor differentiation during CNS remyelination. Proc Natl Acad Sci U S A 106:19162-7 (2009). Mouse. PubMed: 19855010

See all 4 publication references for this product.


If you publish research using ab16794 please let us know so that we can cite the reference on this datasheet.

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