![Western blot - APC antibody [EP701Y] (ab40778) image](#ab40778a.gif)
![Immunohistochemistry (Paraffin-embedded sections) - APC antibody [EP701Y] (ab40778) image](#ab40778b.jpg)
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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-APC antibody [EP701Y]
See all APC products (11) ...
Description
Rabbit monoclonal [EP701Y] to APC
Specificity
This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.
Tested applications
WB, IHC-P, IP, ICCmore details
Cross reactivity
Reacts with
Rat, Human
Does not react with
Mouse
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human APC. The amino acid sequence is not available.
Positive control
293 cell lysate, human colon carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP701Y
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/50.
WB: 1/500 - 1/1000. Detects a band of approximately 160 kDa.
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Cellular localization
Cell junction; adherens junction
Alternative names
- Adenomatous Polyposis Coli antibody
- Adenomatous Polyposis Coli antibody
- APC antibody
see all
Database links
- Entrez Gene: 324 Human
- Entrez Gene: 24205 Rat
- Omim: 175100 Human
- SwissProt: P25054 Human
- SwissProt: P70478 Rat
- Unigene: 88057 Rat
Anti-APC antibody [EP701Y] images:
Western blot - APC antibody [EP701Y] (ab40778)
![Western blot - APC antibody [EP701Y] (ab40778)](/ps/datasheet/Images/40/ab40778/ab40778a.gif)
Anti-APC antibody [EP701Y] (ab40778) at 1/1000 dilution + 10 ug/ml HEK293 cell lysate
Secondary
1:2000 goat anti-rabbit HRP labeled
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - APC antibody [EP701Y] (ab40778)
![Immunohistochemistry (Paraffin-embedded sections) - APC antibody [EP701Y] (ab40778)](/ps/datasheet/Images/40/ab40778/ab40778b.jpg)
ab40778 (1:50), staining human colon carcinoma by immunohistochemistry, paraffin-embedded tissue.
References for Anti-APC antibody [EP701Y] (ab40778)
This product has been referenced in:
- Juhlin CCet al. Parafibromin and APC as screening markers for malignant potential in atypical parathyroid adenomas. Endocr Pathol 21:166-77 (2010). WB, IHC-P; Human.Read more (PubMed: 20473645) »
- Juhlin CCet al. Loss of expression for the Wnt pathway components adenomatous polyposis coli and glycogen synthase kinase 3-beta in parathyroid carcinomas. Int J Oncol 34:481-92 (2009).Read more (PubMed: 19148484) »
See all 2 publications for this product
Publishing research using ab40778? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"