Anti-APC2 antibody (ab113370)
Key features and details
- Rabbit polyclonal to APC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APC2 antibody
See all APC2 primary antibodies -
Description
Rabbit polyclonal to APC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human APC2 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: O95996 -
Positive control
- Human Brain Cortex and Small Intestine tissues; HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa.
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. -
Tissue specificity
Widely expressed (at protein level). Specifically expressed in the CNS. -
Sequence similarities
Belongs to the adenomatous polyposis coli (APC) family.
Contains 6 ARM repeats. -
Developmental stage
Expressed in fetal brain. -
Cellular localization
Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822). - Information by UniProt
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Database links
- Entrez Gene: 10297 Human
- SwissProt: O95996 Human
- SwissProt: Q9Z1K7 Mouse
- Unigene: 446376 Human
- Unigene: 57247 Mouse
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Alternative names
- Adenomatous polyposis coli protein 2 antibody
- Adenomatous polyposis coli protein-like antibody
- APC-like antibody
see all
Images
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ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
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ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.
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Lane 1 : Anti-APC2 antibody (ab113370) at 1 µg/ml
Lane 2 : Anti-APC2 antibody (ab113370) at 2 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 244 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab113370 has been referenced in 3 publications.
- Rashid F et al. Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation. Int J Mol Sci 18:N/A (2017). WB ; Human . PubMed: 28661420
- Najafi H et al. Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway. RNA 23:70-85 (2017). PubMed: 27986894
- Hebert AM et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev 26:2709-23 (2012). WB ; Human . PubMed: 23249734