Anti-APCDD1 antibody (ab73063)
Key features and details
- Mouse polyclonal to APCDD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APCDD1 antibody -
Description
Mouse polyclonal to APCDD1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human APCDD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- APCDD1 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. |
Target
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Function
Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins. May play a role in colorectal tumorigenesis. -
Tissue specificity
Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. Abundantly expressed in both the epidermal and dermal compartments of the hair follicle. Present in scalp skin Highly expressed in the hair follicle dermal papilla, the matrix, and the hair shaft (at protein level). -
Involvement in disease
Defects in APCDD1 are a cause of hypotrichosis simplex (HTS) [MIM:605389]. HTS is a rare form of non-syndromic hereditary hypotrichosis without characteristic hair shaft anomalies. Affected individuals typically show normal hair at birth, but hair loss and thinning of the hair shaft start during early childhood and progress with age. -
Sequence similarities
Belongs to the APCDD1 family. -
Post-translational
modificationsN-Glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 147495 Human
- Omim: 607479 Human
- SwissProt: Q8J025 Human
- Unigene: 293274 Human
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Alternative names
- Adenomatosis polyposis coli down regulated 1 antibody
- Adenomatosis polyposis coli down regulated 1 protein antibody
- Adenomatosis polyposis coli down-regulated 1 protein antibody
see all
Images
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All lanes : Anti-APCDD1 antibody (ab73063) at 1 µg/ml
Lane 1 : APCDD1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 59 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab73063 has been referenced in 1 publication.
- Cho SG APC downregulated 1 inhibits breast cancer cell invasion by inhibiting the canonical WNT signaling pathway. Oncol Lett 14:4845-4852 (2017). PubMed: 29085490