APG5L/ATG5 antibody (ab54033)

Product Name 

APG5L/ATG5 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to APG5L/ATG5

Immunogen 

A synthetic peptide designed within the N-terminal region (aa 1-50) of human APG5L/ATG5.

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, Cow, Mk, Pig, Xl, Zfsh
(Due to sequence homology)

Specificity 

Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/500. Detects a band of approximately 56 kDa (predicted molecular weight: 32 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver; Mouse wildtype ES cell lysate.

Cellular localization 

Cytoplasmic. Colocalizes with nonmuscle actin.

Research areas 

Cancer >> Cancer Metabolism >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cell Biology >> Apoptosis >> Phagocytosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

APG5L is required for autophagy. It conjugates to APG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to APG12 is essential for autophagy, but is not required for association with isolation membrane.
APG5L may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 30% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.170 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for APG5L/ATG5 antibody images, references, products related to ab54033 and other tools.

APG5L/ATG5 antibody images:

References for APG5L/ATG5 antibody (ab54033)

  This product has been used in:

Rossi M et al. Desmethylclomipramine induces the accumulation of autophagy markers by blocking autophagic flux. J Cell Sci 122:3330-9 (2009). WB; Human. PubMed: 19706685

If you publish research using ab54033 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding APG5L/ATG5 antibody

Customer FAQs regarding APG5L/ATG5 antibody

Protocols for APG5L/ATG5 antibody

Price and availability of products related to APG5L/ATG5 antibody

APG5L/ATG5 antibody - related products: