Anti-APG5L/ATG5 antibody (ab64227)

Overview

• Product nameAnti-APG5L/ATG5 antibodySee all APG5L/ATG5 primary antibodies ...
• Description
  Rabbit polyclonal to APG5L/ATG5
• Tested applicationsIHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A synthetic peptide from the internal region of human, mouse and chicken APG5L/ATG5 conjugated to an immunogenic carrier protein

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy
  • APG gene products

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Signal transduction
  • Autophagy

  • Cardiovascular
  • Heart
  • Autophagy
  • APG gene products

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ub-like Proteins

  • Cell Biology
  • Apoptosis
  • Phagocytosis

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionRequired for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.
  May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
• Tissue specificityUbiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
• Sequence similaritiesBelongs to the ATG5 family.
• Post-translational
  modificationsConjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
• Cellular localizationCytoplasm. Colocalizes with nonmuscle actin.

Target information above from: UniProt accession Q9H1Y0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9474 Human
  • Entrez Gene: 11793 Mouse
  • Omim: 604261 Human
  • SwissProt: Q9H1Y0 Human
  • SwissProt: Q99J83 Mouse
  • Unigene: 486063 Human
  • Unigene: 22264 Mouse

• Alternative names
  APG 5 antibodyAPG 5L antibodyAPG5 antibody

  APG5 autophagy 5 like antibodyAPG5 like antibodyAPG5-like antibodyAPG5L antibodyApoptosis specific protein antibodyApoptosis-specific protein antibodyASP antibodyATG 5 antibodyATG5 antibodyATG5 autophagy related 5 homolog antibodyATG5_HUMAN antibodyAutophagy protein 5 antibodyhAPG5 antibodyHomolog of S Cerevisiae autophagy 5 antibodyOTTHUMP00000040507 antibody

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Anti-APG5L/ATG5 antibody images

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  Immunohistochemistry (Frozen sections) - APG5L/ATG5 antibody (ab64227)This image is courtesy of an Abreview submitted by Miss Silke Vorwald

  ab64227 staining mouse Testis tissue sections by immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at   20°C. The primary antibody was diluted 1/800 and incubated with sample for 20 hours at 20°C. The biotin conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary.

  See Abreview