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Western blot - APH1a antibody (ab62521)
(enlarge)
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Product Name
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APH1a antibody - Aminoterminal end
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See all APH1a antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to APH1a - Aminoterminal end
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Immunogen
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Synthetic 13 amino acid peptide from near the amino terminus of human APH1a.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RAW264.7 cell lysate
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
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Research areas
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Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APH1a antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APH1a antibody - Aminoterminal end images, references, products related to ab62521 and other tools.
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APH1a antibody - Aminoterminal end images:
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 Western blot - APH1a antibody (ab62521)
Lane 1 : APH1a antibody - Aminoterminal end (ab62521) at 0.5 µg/ml
Lane 2 : APH1a antibody - Aminoterminal end (ab62521) at 1 µg/ml
Lane 3 : APH1a antibody - Aminoterminal end (ab62521) at 2 µg/ml
Lane 1 : RAW264.7 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 29 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to APH1a
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APH1a antibody - Aminoterminal end - more information
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APH1a antibody - Aminoterminal end - other tools:
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