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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-APH1a antibody - Aminoterminal end
See all APH1a products (6) ...
Description
Rabbit polyclonal to APH1a - Aminoterminal end
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic 13 amino acid peptide from near the amino terminus of human APH1a.
Positive control
RAW264.7 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - APH1a antibody (ab62521)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab62521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Target information above from: UniProt accessionQ96BI3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6530402N02Rik antibody
- AL138795.3 antibody
- Anterior Pharynx Defective 1 antibody
see all
Database links
- Entrez Gene: 51107 Human
- Entrez Gene: 226548 Mouse
- Omim: 607629 Human
- SwissProt: Q5TB23 Human
- SwissProt: Q96BI3 Human
- SwissProt: Q3U886 Mouse
- SwissProt: Q6GTF1 Mouse
- SwissProt: Q8BVF7 Mouse
see all
Anti-APH1a antibody - Aminoterminal end images:
Western blot - APH1a antibody (ab62521)
Lane 1 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 0.5 µg/ml
Lane 2 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 1 µg/ml
Lane 3 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 2 µg/ml
Lane 1 : RAW264.7 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 29 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-APH1a antibody - Aminoterminal end (ab62521)
ab62521 has not yet been referenced specifically in any publications.
Publishing research using ab62521? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"