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Western blot - APH1a antibody - N-terminal (ab60119)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)
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Product Name
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APH1a antibody - N-terminal
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See all APH1a antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to APH1a - N-terminal
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Immunogen
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Synthetic peptide (10-30 aa in length) at the N-terminal of the first 110 amino acids of human APH1a, conjugated to KLH
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
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Research areas
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Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab60119 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APH1a antibody - N-terminal is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APH1a antibody - N-terminal images, references, products related to ab60119 and other tools.
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APH1a antibody - N-terminal images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)
ab60119 at 1/100 dilution staining APH1a in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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Search PubMed (MEDLINE) for references to APH1a
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APH1a antibody - N-terminal - more information
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Customer reviews (feedback) regarding APH1a antibody - N-terminal |
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APH1a antibody - N-terminal - other tools:
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