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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-APH1a antibody - N-terminal
See all APH1a products (6) ...
Description
Rabbit polyclonal to APH1a - N-terminal
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (10-30 aa in length) at the N-terminal of the first 110 amino acids of human APH1a, conjugated to KLH
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab60119 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
IHC-P: 1/50 - 1/100.
Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Target information above from: UniProt accessionQ96BI3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6530402N02Rik antibody
- AL138795.3 antibody
- Anterior Pharynx Defective 1 antibody
see all
Database links
- Entrez Gene: 51107 Human
- Omim: 607629 Human
- SwissProt: Q5TB23 Human
- SwissProt: Q96BI3 Human
- Unigene: 108408 Human
Anti-APH1a antibody - N-terminal images:
Western blot - APH1a antibody - N-terminal (ab60119)
Anti-APH1a antibody - N-terminal (ab60119) at 1/100 dilution
Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)
ab60119 at 1/100 dilution staining APH1a in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-APH1a antibody - N-terminal (ab60119)
ab60119 has not yet been referenced specifically in any publications.
Publishing research using ab60119? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"