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Western blot - APH1a antibody - N-terminal (ab60119)
Western blot - APH1a antibody - N-terminal (ab60119)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)
(enlarge)


Product Name 

APH1a antibody - N-terminal

 

See all APH1a antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to APH1a - N-terminal

Immunogen 

Synthetic peptide (10-30 aa in length) at the N-terminal of the first 110 amino acids of human APH1a, conjugated to KLH

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.

Research areas 

Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway

Relevance 

Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).

 

Alternative names APH1a antibody - N-terminal (ab60119)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51107    (Human)

GeneCard

    

GC01M148504    (Human)

Omim

    

607629    (Human)

SwissProt

    

Q5TB23    (Human)
Q96BI3    (Human)

Unigene

    

108408    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab60119 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APH1a antibody - N-terminal is on this datasheet. But please do contact us if you would like any reassurance!


See below for APH1a antibody - N-terminal images, references, products related to ab60119 and other tools.

 

APH1a antibody - N-terminal images:

Product image  Western blot - APH1a antibody - N-terminal (ab60119)

Western blot - APH1a antibody - N-terminal (ab60119)

APH1a antibody - N-terminal (ab60119) at 1/100 dilution

Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)

ab60119 at 1/100 dilution staining APH1a in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

 
 

Search PubMed (MEDLINE) for references to APH1a

 

APH1a antibody - N-terminal - more information

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APH1a antibody - N-terminal - related products:

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