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Anti-APH1a antibody - N-terminal (ab60119)

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Overview

Product name

Anti-APH1a antibody - N-terminal
See all APH1a products (6) ...

Description

Rabbit polyclonal to APH1a - N-terminal

Tested applications

ELISA, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide (10-30 aa in length) at the N-terminal of the first 110 amino acids of human APH1a, conjugated to KLH

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab60119 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - APH1a antibody - N-terminal (ab60119)Western blot - APH1a antibody - N-terminal (ab60119) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab60119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • ShowHide1 Image

    WB

     WB: 1/100 - 1/500.Detect...Read more →

    WB: 1/100 - 1/500.Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

Tissue specificity

Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.

Sequence similarities

Belongs to the APH-1 family.

Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.

Target information above from: UniProt accessionQ96BI3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 6530402N02Rik antibody
  • AL138795.3 antibody
  • Anterior Pharynx Defective 1 antibody
  • Anterior pharynx defective 1 homolog A antibody
  • APH 1A antibody
  • Aph 1alpha antibody
  • APH-1a antibody
  • Aph-1alpha antibody
  • Aph1a antibody
  • APH1A_HUMAN antibody
  • CGI 78 antibody
  • CGI78 antibody
  • Gamma secretase subunit APH 1A antibody
  • Gamma Secretase Subunit APH1a antibody
  • Gamma-secretase subunit APH-1A antibody
  • Likely ortholog of C. elegans anterior pharynx defective 1A antibody
  • Presenilin Stabilization Factor antibody
  • Presenilin-stabilization factor antibody
  • PSF antibody
  • UNQ579/PRO1141 antibody
see all

Anti-APH1a antibody - N-terminal images:

  Western blot - APH1a antibody - N-terminal (ab60119)

Western blot - APH1a antibody - N-terminal (ab60119)

Anti-APH1a antibody - N-terminal (ab60119) at 1/100 dilution

Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-APH1a antibody - N-terminal(ab60119)

ab60119 at 1/100 dilution staining APH1a in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-APH1a antibody - N-terminal (ab60119)

ab60119 has not yet been referenced specifically in any publications.

Publishing research using ab60119? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"