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Western blot
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Immunohistochemistry (PFA perfusion fixed frozen sections) - APH1a antibody (ab12104)
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Product Name
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APH1a antibody
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See all APH1a antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to APH1a
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Immunogen
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Synthetic peptide derived from residues 50 - 150 of Human APH1a.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Recognises both isoforms of APH1a (encoded on chromosome 1)
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Tested applications
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IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately ~30 kDa (predicted molecular weight: 30 kDa). Can be blocked with APH1a peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
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Research areas
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Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.50 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APH1a antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APH1a antibody images, references, products related to ab12104 and other tools.
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APH1a antibody images:
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 Western blot
All lanes : APH1a antibody (ab12104) at 1/500 dilution
Lane 1 : 20ug human brain lysate
Lane 2 : 20ug mouse brain lysate
Lane 3 : 20ug human brain lysate with APH1a peptide (ab12394) at 1 µg/ml
Lane 4 : 20ug mouse brain lysate with APH1a peptide (ab12394) at 1 µg/ml
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab7090) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Rabbit polyclonal to APH1a (ab12104) used at 1/500 on:
Lane 1: Human Brain Lysate
Lane 2: Mouse Brain Lysate
Lane 3: Human Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)
Lane 4: Mouse Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)
Secondary antibody : Goat polyclonal to Rabbit IgG (HRP) - pre-adsorbed at 1/5000 (ab7090).
Expected molecular weight was 30kDa. The band observed between 20-25kDa is likely to be an isoform of Aph1a.
We are unsure as to the identity of the minor band seen at 75kDa.
Blot exposure time=30 seconds
We do not know the identity of the bands at 21 and 75kDa
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Immunohistochemistry (PFA perfusion fixed frozen sections) - APH1a antibody (ab12104)
ab12104 staining APH1a in mouse brain tissue section (cortex) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. ab12104 produces a very pretty staining in neuronal processes in the cortex and the hippocampus. This image is courtesy of an Abreview submitted by Dr Sophie Pezet
See Abreview
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Search PubMed (MEDLINE) for references to APH1a
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APH1a antibody - more information
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Customer reviews (feedback) regarding APH1a antibody |
Customer FAQs regarding APH1a antibody |
Protocols for APH1a antibody |
Price and availability of products related to APH1a antibody |
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APH1a antibody - related products:
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APH1a antibody - other tools:
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Contact Abcam with a Technical Enquiry about APH1a antibody |
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