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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-APH1a antibody
See all APH1a products (6) ...
Description
Rabbit polyclonal to APH1a
Specificity
Recognises both isoforms of APH1a (encoded on chromosome 1)
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from residues 50 - 150 of Human APH1a.
.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately ~30 kDa (predicted molecular weight: 30 kDa). Can be blocked with APH1a peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Target information above from: UniProt accessionQ96BI3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6530402N02Rik antibody
- AL138795.3 antibody
- Anterior Pharynx Defective 1 antibody
see all
Database links
- Entrez Gene: 51107 Human
- Entrez Gene: 226548 Mouse
- Entrez Gene: 365872 Rat
- Omim: 607629 Human
- SwissProt: Q5TB23 Human
- SwissProt: Q96BI3 Human
- SwissProt: Q3U886 Mouse
- SwissProt: Q6GTF1 Mouse
see all
Anti-APH1a antibody images:
Western blot
All lanes : Anti-APH1a antibody (ab12104) at 1/500 dilution
Lane 1 : 20ug human brain lysate
Lane 2 : 20ug mouse brain lysate
Lane 3 : 20ug human brain lysate with APH1a peptide (ab12394) at 1 µg/ml
Lane 4 : 20ug mouse brain lysate with APH1a peptide (ab12394) at 1 µg/ml
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Rabbit polyclonal to APH1a (ab12104) used at 1/500 on:
Lane 1: Human Brain Lysate
Lane 2: Mouse Brain Lysate
Lane 3: Human Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)
Lane 4: Mouse Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)
Secondary antibody : Goat polyclonal to Rabbit IgG (HRP) - pre-adsorbed at 1/5000 (ab7090).
Expected molecular weight was 30kDa. The band observed between 20-25kDa is likely to be an isoform of Aph1a.
We are unsure as to the identity of the minor band seen at 75kDa.
Blot exposure time=30 seconds
We do not know the identity of the bands at 21 and 75kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - APH1a antibody (ab12104)
ab12104 staining APH1a in mouse brain tissue section (cortex) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. ab12104 produces a very pretty staining in neuronal processes in the cortex and the hippocampus.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet
References for Anti-APH1a antibody (ab12104)
This product has been referenced in:
- Donmez Get al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB; Mouse.Read more (PubMed: 20655472) »
See 1 publication for this product
Publishing research using ab12104? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"