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Western blot - APH1a antibody (ab19390)
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Immunocytochemistry/ Immunofluorescence - APH1a antibody (ab19390)
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Product Name
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APH1a antibody
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See all APH1a antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to APH1a
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Immunogen
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Synthetic peptide: CRRQEDSRVMVYSALRIPPED conjugated to KLH, corresponding to C terminal domain of the long isoform of amino acids 245-265 of Human APH1a.
BLAST 'CRRQEDSRVMVYSALRIPPED' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/100.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 29 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse Brain and Kidney extract (western blot).
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
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Research areas
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Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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Anterior Pharynx Defective 1 (APH1a) is an integral transmembrane protein forming a component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.005% Sodium Azide
Constituents: 16.6% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.690 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APH1a antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APH1a antibody images, references, products related to ab19390 and other tools.
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APH1a antibody images:
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 Western blot - APH1a antibody (ab19390)
Predicted band size : 29 kDa
Western blot of APH1a in mouse brain and kidney extracts using ab19390.
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Immunocytochemistry/ Immunofluorescence - APH1a antibody (ab19390)
Immunofluorescence staining of APH1a in human KNS42 glioma cells using ab19390.
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Search PubMed (MEDLINE) for references to APH1a
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APH1a antibody - more information
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Customer reviews (feedback) regarding APH1a antibody |
Customer FAQs regarding APH1a antibody |
Protocols for APH1a antibody |
Price and availability of products related to APH1a antibody |
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APH1a antibody - related products:
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APH1a antibody - other tools:
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Contact Abcam with a Technical Enquiry about APH1a antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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