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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-APH1a antibody
See all APH1a products (6) ...
Description
Goat polyclonal to APH1a
Specificity
ab62821 is expected to recognize both reported isoforms of APH1a (NP_001071096.1 and NP_057106.2).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: C-HVTDRSDARLQYG, corresponding to internal sequence amino acids 58-70 of Human APH1a
C-HVTDRSDA RLQYG
Positive control
Human Brain (Cerebral Cortex) lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab62821 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - APH1a antibody (ab62821)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: 1/128000.
WB: Use at a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Target information above from: UniProt accessionQ96BI3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6530402N02Rik antibody
- AL138795.3 antibody
- Anterior Pharynx Defective 1 antibody
see all
Database links
- Entrez Gene: 51107 Human
- Entrez Gene: 226548 Mouse
- Entrez Gene: 365872 Rat
- Omim: 607629 Human
- SwissProt: Q5TB23 Human
- SwissProt: Q96BI3 Human
- SwissProt: Q3U886 Mouse
- SwissProt: Q6GTF1 Mouse
see all
Anti-APH1a antibody images:
Western blot - APH1a antibody (ab62821)
Anti-APH1a antibody (ab62821) at 0.05 µg/ml + Brain (Cerebral Cortex) lysate (in RIPA buffer) at 35 µg
Predicted band size : 29 kDa
Observed band size : 29 kDa
References for Anti-APH1a antibody (ab62821)
ab62821 has not yet been referenced specifically in any publications.
Publishing research using ab62821? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"