Anti-APJ Receptor antibody (ab84296)

Overview

  • Product nameAnti-APJ Receptor antibody
    See all APJ Receptor primary antibodies
  • Description
    Rabbit polyclonal to APJ Receptor
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human APJ Receptor.

    (Peptide available as ab101728.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.

Properties

Applications

Our Abpromise guarantee covers the use of ab84296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human APJ Receptor peptide (ab101728).
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
  • Tissue specificityWidely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGTR L1 antibody
    • AGTRL 1 antibody
    • AGTRL1 antibody
    • Angiotensin II receptor like 1 antibody
    • Angiotensin receptor like 1 antibody
    • Angiotensin receptor-like 1 antibody
    • Apelin receptor antibody
    • APJ antibody
    • APJ_HUMAN antibody
    • APLNR antibody
    • B78 antibody
    • FLJ90771 antibody
    • G protein coupled receptor APJ antibody
    • G-protein coupled receptor APJ antibody
    • G-protein coupled receptor HG11 antibody
    • GPCR34 antibody
    • HG 11 antibody
    • HG11 antibody
    • MGC45246 antibody
    • MSR antibody
    • msr/apj antibody
    see all

Anti-APJ Receptor antibody images

  • All lanes : Anti-APJ Receptor antibody (ab84296) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab84296 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84296, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.
  • IHC image of APJ Receptor staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84296, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab84296 staining APJ Receptor in Human endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.2% and blocked with 20% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/300) was used as the secondary antibody.

    See Abreview

References for Anti-APJ Receptor antibody (ab84296)

ab84296 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6.0, 100C 20 minutes
Sample Human Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Oct 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (endothelial cells)
Loading amount 5000 cells
Specification endothelial cells
Gel Running Conditions Reduced Non-Denaturing (Native)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
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Submitted Mar 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (artery)
Specification artery
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
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Abcam user community

Verified customer

Submitted Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (endothelial cell)
Specification endothelial cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
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Verified customer

Submitted Feb 28 2013

Thank you very much for your interest our products. All of the testing data we have is available on the datasheet. So to our knowledge, ab66218 has not been tested in WB and ab84296 has not been tested in rat (although it is predicted to work due to hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"