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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-APLF antibody
See all APLF products (2) ...
Description
Rabbit polyclonal to APLF
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-SP-Q-V).
Positive control
Human brain tissue; HT-29 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
Target
Function
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Sequence similarities
Belongs to the APLF family.
Contains 1 FHA-like domain.
Contains 2 PBZ-type zinc fingers.
Domain
The PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites.
The FHA-like domain mediates interaction with XRCC1 and XRCC4.
Post-translational
modifications
Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.
Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
Cellular localization
Nucleus. Cytoplasm > cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
Target information above from: UniProt accessionQ8IW19
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- XRCC1 interacting protein 1 antibody
- 2010301N04Rik antibody
- AI452191 antibody
see all
Database links
- Entrez Gene: 200558 Human
- Entrez Gene: 72103 Mouse
- Omim: 611035 Human
- SwissProt: Q8IW19 Human
- SwissProt: Q9D842 Mouse
- Unigene: 720369 Human
- Unigene: 31770 Mouse
Anti-APLF antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APLF antibody (ab79371)
ab79371 at 1/50 dilution staining APLF in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - APLF antibody (ab79371)
All lanes : Anti-APLF antibody (ab79371) at 1/500 dilution
Lane 1 : HT-29 cell extracts
Lane 2 : HT-29 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 57 kDa
Observed band size : 57 kDa
References for Anti-APLF antibody (ab79371)
ab79371 has not yet been referenced specifically in any publications.
Publishing research using ab79371? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"