Recombinant Anti-APLP2 antibody [EPR5938(2)] (ab140624)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5938(2)] to APLP2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-APLP2 antibody [EPR5938(2)]
See all APLP2 primary antibodies -
Description
Rabbit monoclonal [EPR5938(2)] to APLP2 -
Host species
Rabbit -
Specificity
This antibody detects isoforms in some cell lines.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human APLP2 aa 200-300. The exact sequence is proprietary.
Database link: Q06481 -
Positive control
- WB: HEK293 cell lysate, and Mouse brain and Rat brain tissue lysates. IHC-P: Human breast carcinoma, Rat cerebrum, and Human cerebrum tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5938(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. We recommend a dilution of 1/500 for human, 1/2000 for rat. For unpurified use at 1/100 - 1/250. |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 87 kDa).
ab140624 detects isoforms in some cell lines |
Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. We recommend a dilution of 1/500 for human, 1/2000 for rat. For unpurified use at 1/100 - 1/250. |
WB
1/10000 - 1/50000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 87 kDa). ab140624 detects isoforms in some cell lines |
Target
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Function
May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. -
Tissue specificity
Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues. -
Sequence similarities
Belongs to the APP family.
Contains 1 BPTI/Kunitz inhibitor domain. -
Post-translational
modificationsThe BPTI/Kunitz inhibitor domain is O-glycosylated. -
Cellular localization
Cell membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 334 Human
- Entrez Gene: 11804 Mouse
- Entrez Gene: 64312 Rat
- Omim: 104776 Human
- SwissProt: Q06481 Human
- SwissProt: Q06335 Mouse
- SwissProt: P15943 Rat
- Unigene: 370247 Human
see all -
Alternative names
- amyloid beta (A4) precursor like protein 2 antibody
- Amyloid like protein 2 antibody
- Amyloid precursor protein homolog HSD 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling APLP2 with purified ab140624 at 1:500 (0.188 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as a reporter at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/10000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 16279945).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling APLP2 with purified ab140624 at 1:2000 (0.047 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling APLP2 with purified ab140624 at 1:500 (0.188 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab140624 has been referenced in 1 publication.
- Currinn H et al. APP controls the formation of PI(3,5)P(2) vesicles through its binding of the PIKfyve complex. Cell Mol Life Sci 73:393-408 (2016). PubMed: 26216398