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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Overview
Product name
Anti-APLP2 antibody
See all APLP2 products (3) ...
Description
Rabbit polyclonal to APLP2
Specificity
Does not cross-react with APP and APLP1.
Tested applications
IHC-P, WB, IP, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length APLP2 protein (Mouse)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration.
WB: 1/5000 - 1/20000.Predicted molecular weight: 87 kDa.
IP: Use at 1-2 µg/mg of lysate.
IHC-Fr
IHC-Fr: 1/1000IHC-Fr: 1/1000
IHC-P: 1/1000
ICC/IF
ICC/IF: 1/250 - 1/500.ICC/IF: 1/250 - 1/500.
Target
Function
May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein.
Tissue specificity
Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
Sequence similarities
Belongs to the APP family.
Contains 1 BPTI/Kunitz inhibitor domain.
Post-translational
modifications
The BPTI/Kunitz inhibitor domain is O-glycosylated.
Cellular localization
Cell membrane. Nucleus.
Target information above from: UniProt accessionQ06481
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- amyloid beta (A4) precursor like protein 2 antibody
- Amyloid like protein 2 antibody
- amyloid precursor protein homolog HSD2 antibody
see all
Database links
- Entrez Gene: 334 Human
- Entrez Gene: 11804 Mouse
- Entrez Gene: 64312 Rat
- SwissProt: Q06481 Human
- SwissProt: Q06335 Mouse
- SwissProt: P15943 Rat
- Unigene: 370247 Human
- Unigene: 695920 Human
see all
Anti-APLP2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APLP2 antibody (ab58583)
Immunohistochemistical detection of APLP2 using antibody ab58583 on (Formaldehyde-fixed paraffin-embedded rat lumbar DRG sections). Antigen retrieval step: heat mediated; Buffer used: Citric acid pH6; Blocking step: 1% BSA for 10 mins@ rt°C. Primary antibody ab58583 diluted @ 1/1000, incubated for 16 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG conjugated to biotin (1/200). The immunostaining appears to be localised to the cytoplasm: a widely distributed punctate positivity is seen in DRG cells. Note that DRG nuclei are "absent": Citric acid HIER pH6, in my hands, tends to hydrolyse DNA in highly metabolically active cells. NB: other nuclei are very well stained. I overstained this section with Hx: note the blue staining of the myelin sheaths.
Carl Hobbs, King`s College London, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APLP2 antibody (ab58583)
Immunohistochemistical detection of APLP2 using antibody ab58583 on formaldehyde-fixed paraffin-embedded mouse TASTPM mouse brain (end-stage) sections. Antigen retrieval step: Heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA for 10 mins @ RT°C. Primary antibody ab58583 used at 1/2500 incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgGs conjugated to biotin (1/200). When I probed this Ab on TASTPM brain I found a very high elevation of positivity in amyloid plaques (the same in Tg2576 mouse brain). The lower image is included to confirm that APLP2 is demonstrated in amyloid plaques: both images were taken using the same capture conditions.
Carl Hobbs, King`s College London, United Kingdom
References for Anti-APLP2 antibody (ab58583)
ab58583 has not yet been referenced specifically in any publications.
Publishing research using ab58583? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"