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Overview

  • Product nameAnti-APOA4 antibodySee all APOA4 primary antibodies ...
  • Description
    Goat polyclonal to APOA4
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Baboon, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: C-KEKESQDKTLSL, corresponding to internal sequence amino acids 359-370 of Human APOA4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman small intestine tissue; Human duodenum lysate

Applications

Our Abpromise guarantee covers the use of ab115308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 45 kDa.
ELISA ELISA: 1/32000.
IHC-P IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceAPOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • Cellular localizationSecreted
  • Database links
    • Alternative names
        Apo AIV antibodyAPOA 4 antibodyApoA IV antibody
        APOA4 antibodyApolipoprotein A IV antibodyApolipoprotein A4 antibodyMGC142154 antibodyMGC142156 antibody
      see all

    Anti-APOA4 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4 antibody (ab115308)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4 antibody (ab115308)
      ab115308 at 5ug/ml staining APOA4 in Human small intestine tissue by immunohistochemistry (FFPE). Slides were subject to heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with ab115308 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
    • Western blot - Anti-APOA4 antibody (ab115308)
      Western blot - Anti-APOA4 antibody (ab115308)
      Anti-APOA4 antibody (ab115308) at 0.01 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg
      developed using the ECL technique

      Predicted band size : 45 kDa

    References for Anti-APOA4 antibody (ab115308)

    ab115308 has not yet been referenced specifically in any publications.

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