Overview

  • Product nameAnti-APOA4 antibodySee all APOA4 primary antibodies ...
  • Description
    Goat polyclonal to APOA4
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Baboon, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: C-KEKESQDKTLSL, corresponding to internal sequence amino acids 359-370 of Human APOA4

  • Positive control
    • Human small intestine tissue; Human duodenum lysate

Applications

Our Abpromise guarantee covers the use of ab115308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 45 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceAPOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Apo AIV antibody
    • APOA 4 antibody
    • ApoA IV antibody
    • APOA4 antibody
    • Apolipoprotein A IV antibody
    • Apolipoprotein A4 antibody
    • MGC142154 antibody
    • MGC142156 antibody
    see all

Anti-APOA4 antibody images

  • ab115308 at 5ug/ml staining APOA4 in Human small intestine tissue by immunohistochemistry (FFPE). Slides were subject to heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with ab115308 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Anti-APOA4 antibody (ab115308) at 0.01 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg
    developed using the ECL technique

    Predicted band size : 45 kDa

References for Anti-APOA4 antibody (ab115308)

ab115308 has not yet been referenced specifically in any publications.

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