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Western blot - APOBEC1 antibody (ab80493)

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Product Name
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APOBEC1 antibody
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See all APOBEC1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to APOBEC1
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Immunogen
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Synthetic peptide: DVFYDPRELRKEAC, corresponding to internal sequence amino acids 24-37 of Human APOBEC1 (NP_001635.2). BLAST 'DVFYDPRELRKEAC' with or 
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Reacts with
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Ms
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Predicted to react
(species key)
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Hu, Orngt (Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Peptide ELISA: antibody detection limit dilution 1/32000. WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse spleen lysate.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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APOBEC1 is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Interacts with HNRPAB and SYNCRIP.
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APOBEC1 antibody (ab80493)
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APOBEC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for APOBEC1 antibody images, references, products related to ab80493 and other tools.
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APOBEC1 antibody images:
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Western blot - APOBEC1 antibody (ab80493)
APOBEC1 antibody (ab80493) at 1 µg/ml + mouse spleen lysate (in RIPA buffer) at 35 µg
Predicted band size : 28 kDa Observed band size : 28 kDa
Primary incubation was 1 hour.
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Search PubMed (MEDLINE) for references to APOBEC1
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APOBEC1 antibody - more information
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Customer reviews (feedback) regarding APOBEC1 antibody |
Customer FAQs regarding APOBEC1 antibody |
Protocols for APOBEC1 antibody |
Price and availability of products related to APOBEC1 antibody |
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APOBEC1 antibody - related products:
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APOBEC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about APOBEC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"