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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-APOBEC1 antibody
See all APOBEC1 products (2) ...
Description
Goat polyclonal to APOBEC1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human, Orangutan
Immunogen
Synthetic peptide: DVFYDPRELRKEAC, corresponding to internal sequence amino acids 24-37 of Human APOBEC1 (NP_001635.2).
DVFYDPRELR KEAC
Positive control
Mouse spleen lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Western blot - APOBEC1 antibody (ab80493)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA.
Tissue specificity
Expressed exclusively in the small intestine.
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family.
Target information above from: UniProt accessionP41238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABEC1_HUMAN antibody
- APOBEC 1 antibody
- APOBEC1 antibody
see all
Database links
- Entrez Gene: 339 Human
- Entrez Gene: 11810 Mouse
- GenBank: L25877 Human
- GenBank: U21951 Mouse
- Omim: 600130 Human
- SwissProt: P41238 Human
- SwissProt: P51908 Mouse
- SwissProt: Q3TLN2 Mouse
see all
Anti-APOBEC1 antibody images:
Western blot - APOBEC1 antibody (ab80493)
Anti-APOBEC1 antibody (ab80493) at 1 µg/ml + mouse spleen lysate (in RIPA buffer) at 35 µg
Predicted band size : 28 kDa
Observed band size : 28 kDa
Primary incubation was 1 hour.
References for Anti-APOBEC1 antibody (ab80493)
ab80493 has not yet been referenced specifically in any publications.
Publishing research using ab80493? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"