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Western blot - APOBEC1 antibody (ab80493)
Western blot - APOBEC1 antibody (ab80493)
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Product Name 

APOBEC1 antibody

 

See all APOBEC1 antibodies (3)...

Product type 

Primary antibodies

Description 

Goat polyclonal to APOBEC1

Immunogen 

Synthetic peptide: DVFYDPRELRKEAC, corresponding to internal sequence amino acids 24-37 of Human APOBEC1 (NP_001635.2).
BLAST 'DVFYDPRELRKEAC' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Orngt
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse spleen lysate.

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other

Relevance 

APOBEC1 is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Interacts with HNRPAB and SYNCRIP.

 

APOBEC1 antibody (ab80493)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

339    (Human)
11810    (Mouse)

GenBank

    

L25877    (Human)
U21951    (Mouse)

GeneCard

    

GC12M007693    (Human)

Omim

    

600130    (Human)

SwissProt

    

P41238    (Human)
P51908    (Mouse)
Q3TLN2    (Mouse)
Q3U9G8    (Mouse)
Q694B3    (Orangutan)

Unigene

    

560    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APOBEC1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for APOBEC1 antibody images, references, products related to ab80493 and other tools.

 

APOBEC1 antibody images:

Product image  Western blot - APOBEC1 antibody (ab80493)

Western blot - APOBEC1 antibody (ab80493)

APOBEC1 antibody (ab80493) at 1 µg/ml + mouse spleen lysate (in RIPA buffer) at 35 µg

Predicted band size : 28 kDa
Observed band size : 28 kDa


Primary incubation was 1 hour.

 
 

Search PubMed (MEDLINE) for references to APOBEC1

 

APOBEC1 antibody - more information

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APOBEC1 antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for APOBEC1 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other APOBEC1 Antibodies

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APOBEC1 antibody - other tools:

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