Anti-APOBEC3B antibody (ab222330)
Key features and details
- Rabbit polyclonal to APOBEC3B
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOBEC3B antibody
See all APOBEC3B primary antibodies -
Description
Rabbit polyclonal to APOBEC3B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human APOBEC3B aa 250-350.
Database link: Q9UH17 -
Positive control
- ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Relevance
APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9582 Human
- Omim: 607110 Human
- SwissProt: Q9UH17 Human
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Alternative names
- A3B antibody
- APOBEC1L antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab222330 has been referenced in 1 publication.
- Gara SK et al. GATA3 and APOBEC3B are prognostic markers in adrenocortical carcinoma and APOBEC3B is directly transcriptionally regulated by GATA3. Oncotarget 11:3354-3370 (2020). PubMed: 32934779