Anti-APOBEC3G/A3G antibody (ab109727)
Key features and details
- Goat polyclonal to APOBEC3G/A3G
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOBEC3G/A3G antibody
See all APOBEC3G/A3G primary antibodies -
Description
Goat polyclonal to APOBEC3G/A3G -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human APOBEC3G/A3G aa 350-450 (C terminal).
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Positive control
- Daudi cell lysate, Human kidney and Human testis tissues
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. -
Tissue specificity
Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. -
Post-translational
modificationsUbiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF. - Information by UniProt
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Database links
- Entrez Gene: 200316 Human
- Entrez Gene: 60489 Human
- Omim: 607113 Human
- SwissProt: Q9HC16 Human
- Unigene: 659809 Human
- Unigene: 660143 Human
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Alternative names
- A3G antibody
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
see all
Images
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Anti-APOBEC3G/A3G antibody (ab109727) at 0.5 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 46 kDa
Primary incubation was 1 hour -
ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Kidney, Formalin-Fixed, Paraffin-Embedded section.
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ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Testis, Formalin-Fixed, Paraffin-Embedded section.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab109727 has been referenced in 1 publication.
- Evangelou K et al. Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis. Eur Respir J 60:N/A (2022). PubMed: 35086840